%0 Journal Article %T Effect of Pediococcus acidilactici probiotic and Agaricus bisporus prebiotic supplements on the expression of immune-related genes in common carp (Cyprinus carpio) exposed to silver nanoparticles %J Aquatic Physiology and Biotechnology %I University of Guilan %Z 2345-3966 %A Khaleghi, Seyed Reza %A Hedayati, Seyed Ali Akbar %A Kashiri, Hadise %A Paknejad, Hamed %A Hosseinifar, Seyed Hossein %D 2019 %\ 02/20/2019 %V 6 %N 4 %P 155-174 %! Effect of Pediococcus acidilactici probiotic and Agaricus bisporus prebiotic supplements on the expression of immune-related genes in common carp (Cyprinus carpio) exposed to silver nanoparticles %K Nanoparticles %K Common carp %K Gene TNF-1α %K IL-8 %K LYZ-c %R 10.22124/japb.2019.9818.1233 %X The aim of this study was to evaluate the effects of food supplements Pediococcus acidilactici probiotic and Agaricus bisporus prebiotic separate and together (synbiotics) on the expression of immune-mediated genes (TNF-1α, IL-8 and LYZ-c) in common carp exposed to silver nanoparticles. For this aim, 180 carp with an average weight of 29.6±0.4g were divided into four groups of probiotic, prebiotic, combined and control (feeding with no supplements) and were fed for 8 weeks. After the end of feeding period, the fish were exposed to the concentration of sub-acute nano-silver (1mg/L) for 14 days. Sampling from the intestinal tissue was performed to measure the expression of these genes during days 1, 7 and 14. The expression of TNF-1α gene increased significantly with probiotic and periotic dietary supplementation during increasing days of exposure to nano-silver (P<0.05).The expression level of this gene was increased in the 14 days treatments (1.61) compared to other treatments. Expression of IL-8 and LYZ-c genes was highest on 1st day of exposure. However, on the 7th and 14th days of exposure to the nano-silver, the expression of these two genes decreased significantly (P<0.05). However, in spite of the use of synbiotics supplementation in diet, it was not superior to their separate use. Separate or combined use of P. acidilactici as a probiotic and A. bisporus as a prebiotic in the diet can increase the relative expression of TNF-1α gene in common carp exposed to nanosilver. Also, these supplements did not affect the expression of IL-8 and LYZ-c genes. %U https://japb.guilan.ac.ir/article_3338_1f06e9b26b0b71caad6751504085166e.pdf