TY - JOUR ID - 5021 TI - Effects of diets containing Lactococcus lactis and Bacillus subtilis on growth indices and hepatic enzymes of tiger oscar (Astronotus ocellatus) JO - Aquatic Physiology and Biotechnology JA - JAPB LA - en SN - 2345-3966 AU - Hasaninia, Abbas AU - Vahabzadeh, Habib AU - Khara, Hossein AU - Shenavar-e-Masouleh, Alireza AU - Ahmadnezhad, Mohaddeseh AD - Ph.D. Student in Fisheries, Department of Fisheries, Lahijan Branch, Islamic Azad University, Lahijan, Iran AD - Assisstant Proffessor in Department of Fisheries, Lahijan Branch, Islamic Azad University, Lahijan, Iran AD - Associate Professor in Department of Fisheries, Lahijan Branch, Islamic Azad University, Lahijan, Iran AD - Assistant Professor in International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran AD - Assistant Professor in Inland Waters Aquaculture Research Center, Iranian Fisheries Sciences Research, Agricultural Research, Education and Extension Organization (AREEO), Bandar Anzali, Iran Y1 - 2021 PY - 2021 VL - 9 IS - 2 SP - 39 EP - 58 KW - probiotic KW - Growth KW - Tiger Oscar KW - Bacteria KW - Hepatic enzymes DO - 10.22124/japb.2021.17971.1398 N2 - Tiger oscar (Astronotus ocellatus) is a favorite for aquarium fish enthusiasts due to its beauty and compatibility. The study was conducted to evaluate the effects of Lactococcus lactis and Bacillus subtilis on growth efficacy and hepatic enzymes of oscar by selecting 300 juveniles with a mean weight of 8.96±0.03g and length of 8.23±0.02cm. During 70 days fishes fed by diets contained L. lactis and B. subtilis in 9 single and combined treatments groups with the number 1010CFU/g bacteria in treatments which included 150, 300, and 450mg/kg of L. lactis (LL150, LL300, and LL450), 150, 300, and 450 mg/kg of B. subtilis (BS150, BS300, and BS450), 150, 300, and 450 mg/kg of an equal mixture of L. lactis and B. subtilis (MIX150, MIX300, and MIX450) and control group. Finally, their growth indices and hepatic enzymes were assessed. Results showed that applying both bacteria in the feeding of oscar fish has significantly improved the growth indices. Also, adding bacteria in the diet led to increased weight gain (WG), body weight index (BWI), average daily growth (ADG), Specific growth rate (SGR), final biomass and protein efficiency ratio (PER) compared to control (p < 0.05). All single and combined treatments compared to control had the most reduction of FCR which was statistically significant (p < 0.05). Also, the highest statistically significant difference between hepatic enzymes, alanine aminotransferase and aspartate aminotransferase was measured with control in treatment 3 (LL450) and alkaline phosphatase in treatment 9 (MIX450) (p < 0.05). The addition of these bacteria individually or in combination had a positive effect on growth indices and liver enzymes. UR - https://japb.guilan.ac.ir/article_5021.html L1 - https://japb.guilan.ac.ir/article_5021_dd2004e109dc05ed44deb8a965d85b1a.pdf ER -