University of Guilan Aquatic Physiology and Biotechnology 2345-3966 5 2 2017 08 23 Effects of different levels of diet nano selenium (Nano-Se) on growth and gonad quality indices and seminal plasma antioxidants in male goldfish (Carassius auratus gibelio) Effects of different levels of diet nano selenium (Nano-Se) on growth and gonad quality indices and seminal plasma antioxidants in male goldfish (Carassius auratus gibelio) 67 82 2406 10.22124/japb.2017.2406 FA Javad Seiedi M.Sc. in Fisheries, Department of Aquaculture, Marine Sciences Faculty, Tarbiat Modares University, Noor, Iran Mohammad Reza Kalbassi Professor in Department of Aquaculture, Marine Sciences Faculty, Tarbiat Modares University, Noor, Iran Journal Article 2016 10 22 Successful reproduction is one of the most important issues in aquaculture. In this regard, gamete quality is a limiting factor. Selenium, improves the reproduction and immunity in all animals. In the present study, the effects of diets containing different level of Nano-Se on growth indices, gonad histology and activity of two seminal plasma antioxidants, malondialdehyde (MDA) and glutathione peroxidase (GPx), were assessed. Male goldfish (67/28±0/74g, n=200) were fed with 0 (control), 0.1, 0.5 and 1 mg of Nano-Se per Kg of a commercial food for 8 weeks. The developmental stages of the gonadal were evaluated using a common histological method. HCG hormone injection was done to artificially obtain the sperm. The higher Nano-Se concentrations increased and decreased the growth and FCR, respectively. The activity of GPx and MDA in seminal plasma increased in the fishes fed the diets containing Nano-Se, and also the histological observations of the gonads illustrated an increase in spermatozoa count. Hence, 0.5 and 1 mg/Kg Nano-Se improve the growth and gonad indices, and increase the activity of the antioxidant seminal plasma enzymes in goldfish. Successful reproduction is one of the most important issues in aquaculture. In this regard, gamete quality is a limiting factor. Selenium, improves the reproduction and immunity in all animals. In the present study, the effects of diets containing different level of Nano-Se on growth indices, gonad histology and activity of two seminal plasma antioxidants, malondialdehyde (MDA) and glutathione peroxidase (GPx), were assessed. Male goldfish (67/28±0/74g, n=200) were fed with 0 (control), 0.1, 0.5 and 1 mg of Nano-Se per Kg of a commercial food for 8 weeks. The developmental stages of the gonadal were evaluated using a common histological method. HCG hormone injection was done to artificially obtain the sperm. The higher Nano-Se concentrations increased and decreased the growth and FCR, respectively. The activity of GPx and MDA in seminal plasma increased in the fishes fed the diets containing Nano-Se, and also the histological observations of the gonads illustrated an increase in spermatozoa count. Hence, 0.5 and 1 mg/Kg Nano-Se improve the growth and gonad indices, and increase the activity of the antioxidant seminal plasma enzymes in goldfish. https://japb.guilan.ac.ir/article_2406_7d22bf2802addb3101b0e2dd64e22cc4.pdf