University of Guilan Aquatic Physiology and Biotechnology 2345-3966 6 4 2019 02 20 Investigation on genetic diversity of Epeorus along Gamasiab River in different seasons Investigation on genetic diversity of Epeorus along Gamasiab River in different seasons 1 20 3299 10.22124/japb.2019.9095.1207 FA Lima Tayebi Assistant Professor in Department of Fisheries, Faculty of Environmemt and Natural Resources, Malayer University, Malayer, Iran Hadi Poorbagher Associate Professor in Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran Hamid Farahmand Professor in Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran Journal Article 2017 11 22 This study was conducted to determine the profile of Epeorus genetic diversity along the Gamasiab River. The sampling was done from four stations ranged source of the river downward, about 500 meters from each other, in four seasons with three replications from the middle and riverside of the river by the Surber sampler. After isolation and fixation of the Epeorus, the DNA extraction steps were performed and the quality of DNA was investigated on the electrophoresis gel. After gene amplification using specific cytochrome oxidase subunit I (COI) primers, PCR products were sequenced. Sequencing was performed with MEGA 6 software. Molecular investigations indicated that the reduction of genetic diversity at station 2 exposed to wastewater and trout farm effluents, especially in the seasons which are associated with decreasing water charge. This study was conducted to determine the profile of Epeorus genetic diversity along the Gamasiab River. The sampling was done from four stations ranged source of the river downward, about 500 meters from each other, in four seasons with three replications from the middle and riverside of the river by the Surber sampler. After isolation and fixation of the Epeorus, the DNA extraction steps were performed and the quality of DNA was investigated on the electrophoresis gel. After gene amplification using specific cytochrome oxidase subunit I (COI) primers, PCR products were sequenced. Sequencing was performed with MEGA 6 software. Molecular investigations indicated that the reduction of genetic diversity at station 2 exposed to wastewater and trout farm effluents, especially in the seasons which are associated with decreasing water charge.

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