University of Guilan Aquatic Physiology and Biotechnology 2345-3966 8 1 2020 06 13 Protective effect of dietary vitamin E and nano-selenium supplementations on growth performance and hematological parameters of rainbow trout (Oncorhynchus mykiss) exposed to sublethal level of ammonia Protective effect of dietary vitamin E and nano-selenium supplementations on growth performance and hematological parameters of rainbow trout (Oncorhynchus mykiss) exposed to sublethal level of ammonia 95 122 4090 10.22124/japb.2020.12475.1311 FA Afieh Nazer M.Sc. student of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad-Kavous University, Gonbad-Kavous, Iran. Mohammad Harsij Assistant Professor in Department of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad-Kavous University, Gonbad-Kavous, Iran Seyedeh Ainaz Shirangi Assistant Professor in Department of Biology, Faculty of Science and Engineering, Gonbad-Kavous University, Gonbad-Kavous, Iran Hossein Adineh Assistant Professor in Department of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad-Kavous University, Gonbad-Kavous, Iran Journal Article 2019 02 02 This study was performed to improve the physiological responses of rainbow trout to chronic stress of ammonia, as an inhibiting factor in recirculating aquaculture system, by using dietary antioxidant. 150 fish (initial mean weight of 8.4±0.84 g) were investigated in five treatments, each with three replicates for 60 days including fish fed with no supplemented food without ammonia exposure (C^-), without supplemented food but with ammonia exposure (C⁺), with supplementation of vitamin E and ammonia exposure, with supplementation of nano-selenium and ammonia exposure (Se) and with supplementation of combined vitamin E and nano-selenium with ammonia exposure (E+Se). After 30 days of feeding with supplements, the fish were exposed to the ammonia (NH₄OH: 0.2 ppm) for 30 days. Growth and hematological parameters were studied at days of 44 and 60. Results showed that feeding fishes with E+Se increased the growth parameters and reduced plasma ammonia compared to other treatments. Fish fed with E+Se had the highest RBC, hemoglobin and hematocrit levels while WBC was not significant compared to C^- group. These results indicated the synergistic effects of dietary E+Se supplement in rainbow trout fish, it could compensate the imposed damaged by ammonia pollutions. This study was performed to improve the physiological responses of rainbow trout to chronic stress of ammonia, as an inhibiting factor in recirculating aquaculture system, by using dietary antioxidant. 150 fish (initial mean weight of 8.4±0.84 g) were investigated in five treatments, each with three replicates for 60 days including fish fed with no supplemented food without ammonia exposure (C^-), without supplemented food but with ammonia exposure (C⁺), with supplementation of vitamin E and ammonia exposure, with supplementation of nano-selenium and ammonia exposure (Se) and with supplementation of combined vitamin E and nano-selenium with ammonia exposure (E+Se). After 30 days of feeding with supplements, the fish were exposed to the ammonia (NH₄OH: 0.2 ppm) for 30 days. Growth and hematological parameters were studied at days of 44 and 60. Results showed that feeding fishes with E+Se increased the growth parameters and reduced plasma ammonia compared to other treatments. Fish fed with E+Se had the highest RBC, hemoglobin and hematocrit levels while WBC was not significant compared to C^- group. These results indicated the synergistic effects of dietary E+Se supplement in rainbow trout fish, it could compensate the imposed damaged by ammonia pollutions. https://japb.guilan.ac.ir/article_4090_7f997a2f1bc58fff9bdee2cdc99c496e.pdf