University of Guilan Aquatic Physiology and Biotechnology 2345-3966 8 8 2020 11 21 Evaluation of antibacterial and antibiofilm activity of phases extracted from coelomic fluid of burrowing urchin (Echinometra mathaei) Evaluation of antibacterial and antibiofilm activity of phases extracted from coelomic fluid of burrowing urchin (<i>Echinometra mathaei</i>) 145 174 4479 10.22124/japb.2020.14227.1345 FA Majid Mosapour Shajani Ph.D. Student in Fisheries-Seafood Processing, Department of Seafood Processing, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University, Noor, Iran Seyyed Fakhreddin Hosseini Associate Professor in Department of Seafood Processing, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University, Noor, Iran Masoud Rezaei Professor in Department of Seafood Processing, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University, Noor, Iran Domenico Schillaci Associate Professor in Department of Biological, Faculty of Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Palermo, Italy Journal Article 2019 08 27 Marine echinoderms, including sea urchins, have a high ability to survive due to living in shallow environments that are suitable for the growth of microbes. Due to such unique characteristics, much attention has been paid to the purification and identification of a variety of bioactive compounds, especially antimicrobial peptides. Therefore, the present study aimed to investigate the coelomic fluid of the burrowing urchin (<em>Echinometra mathaei</em>), as the most abundant species in the Persian Gulf, and to extract the aqueous and organic phases from coelomocyte cells by a particular solvent. The results of the agar disk and well diffusion assays showed that the organic phase had a higher antibacterial activity, which could be attributed to its protein content. The organic phase at a concentration of higher than 12.5mg.mL^-1 caused complete inhibition of the bacterial biofilm, while it destroyed the biofilm completely in the concentration of 100mg.ml^-1. The organic phase lacked haemolytic activity at the concentration of 100mg.ml^-1 and even inhibited the red blood cell haemolysis. Based on the results, it is suggested that the organic phase extracted from the burrowing urchin coelomocyte may be considered for the extraction and identification of antimicrobial compounds. Marine echinoderms, including sea urchins, have a high ability to survive due to living in shallow environments that are suitable for the growth of microbes. Due to such unique characteristics, much attention has been paid to the purification and identification of a variety of bioactive compounds, especially antimicrobial peptides. Therefore, the present study aimed to investigate the coelomic fluid of the burrowing urchin (<em>Echinometra mathaei</em>), as the most abundant species in the Persian Gulf, and to extract the aqueous and organic phases from coelomocyte cells by a particular solvent. The results of the agar disk and well diffusion assays showed that the organic phase had a higher antibacterial activity, which could be attributed to its protein content. The organic phase at a concentration of higher than 12.5mg.mL^-1 caused complete inhibition of the bacterial biofilm, while it destroyed the biofilm completely in the concentration of 100mg.ml^-1. The organic phase lacked haemolytic activity at the concentration of 100mg.ml^-1 and even inhibited the red blood cell haemolysis. Based on the results, it is suggested that the organic phase extracted from the burrowing urchin coelomocyte may be considered for the extraction and identification of antimicrobial compounds. https://japb.guilan.ac.ir/article_4479_31d4d1b9bfccdbcc638af1429e8abe6f.pdf