University of Guilan Aquatic Physiology and Biotechnology 2345-3966 8 4 2021 02 19 Screening of epidermal mucus from Neogobius fluviatilis pallasi for finding antimicrobial peptides Screening of epidermal mucus from Neogobius fluviatilis pallasi for finding antimicrobial peptides 93 114 4689 10.22124/japb.2021.15917.1371 FA Mohammad Akhavan Bahabadi Ph.D. in Aquaculture, Department of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran Hamed Paknejad Associate Professor in Department of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran Mehran Habibi Rezaei Professor in Department of Cell and Molecular Biology, Protein Biotechnology Research Lab (PBRL), School of Biology, University of Tehran, Tehran, Iran Aliakbar Hedayati Associate Professor in Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran Hamid Moghimi Associate Professor in Department of Microbiology, School of Biology, University of Tehran, Tehran, Iran Journal Article 2020 03 05 In recent years the advent and spread of diverse multidrug-resistant bacteria have been a main object of concern to the medical field and the public health. Antimicrobial peptides (AMPs) are short, usually positive charge and hydrophobic molecules observed in an extensive array of life forms from prokaryotes to eukaryotes including humans. Introducing the group of molecules has been promising as that has some benefits over the significant world well-being concerns. This research describes the antimicrobial activity of the epidermal exudates extract of Neogobius fluviatilis pallasi and its size-based fractionations for the first time. The results showed that epidermal mucus extract and its fractionations were toxic to Bacillus subtilis and Staphylococcus aureus with radial of inhibition 12.25 and 19.25 mm, respectively. However, Pseudomonas aeruginosa, Escherichia coli, Vibrio harveyi, Yersinia ruckeri were resistant. Proteolytic treatment with proteinase K led to depleting the antibacterial properties of fractions, indicating that the active agent possess a proteinaceous nature. Overall, these results propose that fish skin mucus is a rich and valuable source for finding novel AMPs with potential applications in aquaculture and medicine. In recent years the advent and spread of diverse multidrug-resistant bacteria have been a main object of concern to the medical field and the public health. Antimicrobial peptides (AMPs) are short, usually positive charge and hydrophobic molecules observed in an extensive array of life forms from prokaryotes to eukaryotes including humans. Introducing the group of molecules has been promising as that has some benefits over the significant world well-being concerns. This research describes the antimicrobial activity of the epidermal exudates extract of Neogobius fluviatilis pallasi and its size-based fractionations for the first time. The results showed that epidermal mucus extract and its fractionations were toxic to Bacillus subtilis and Staphylococcus aureus with radial of inhibition 12.25 and 19.25 mm, respectively. However, Pseudomonas aeruginosa, Escherichia coli, Vibrio harveyi, Yersinia ruckeri were resistant. Proteolytic treatment with proteinase K led to depleting the antibacterial properties of fractions, indicating that the active agent possess a proteinaceous nature. Overall, these results propose that fish skin mucus is a rich and valuable source for finding novel AMPs with potential applications in aquaculture and medicine. https://japb.guilan.ac.ir/article_4689_bfb0f45e49dc243ebc91f561802b0378.pdf