University of Guilan Aquatic Physiology and Biotechnology 2345-3966 11 1 2023 06 21 The effect of probiotic bacteria Bacillus subtilis and Lactobacillus plantarum on fungal control and egg hatching performance of rainbow trout (Oncorhynchus mykiss) The effect of probiotic bacteria <i>Bacillus subtilis</i> and <i>Lactobacillus plantarum</i> on fungal control and egg hatching performance of rainbow trout (<i>Oncorhynchus mykiss</i>) 105 125 6853 10.22124/japb.2022.22297.1465 FA Omid Rahmati Associate Professor in Department of Fisheries, Faculty of Natural Resources, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran Saeid Aeyaeinejad Associate Professor in Department of Fisheries, Faculty of Natural Resources, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran Ali Abromand Associate Professor in Department of Fisheries, Faculty of Natural Resources, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran Esmaeil Kazemi Ph.D. in Fisheries, Fisheries Research Institute of Iran, Yasouj Shahid Motahari Clod-water Fish Genetics and Breeding Research Center, Fisheries Research Institute of Iran, Yasouj, Iran Journal Article 2022 05 15 In the fish breeding industry, many disinfectants are used to prevent fungal contamination of eggs during the incubation period, including formalin, hydrogen peroxide, potassium permanganate, etc. The purpose of this research is to evaluate the effect of probiotic bacteria Bacillus subtilis 1 and 2 grams and Lactobacillus plantarum 1 and 2 grams (adding to water) in controlling the fungal contamination of rainbow trout eggs during the incubation stage and comparing its effects with formalin and It was distilled water. To conduct this study, 18 Californian troughs were used in 6 treatments and three repetitions, and 1850 fertilized rainbow trout eggs were transferred to each trough. After preparing the trays and distributing the eggs, a separate serum was used for each treatment to disinfect the eggs. The duration of complete emptying of each serum was 50 minutes. The results showed that in the treatments containing formalin (10±1) followed by Lactobacillus 2 grams (15.33±3.21), the lowest rate of fungal infection of eggs was observed, which showed a significant difference with other treatments. P>0.05). The highest rate of eye opening (84.67±0.58) and egg opening (81±1) was observed in formalin treatment, which was significantly different from other treatments (P<0.05) In the fish breeding industry, many disinfectants are used to prevent fungal contamination of eggs during the incubation period, including formalin, hydrogen peroxide, potassium permanganate, etc. The purpose of this research is to evaluate the effect of probiotic bacteria Bacillus subtilis 1 and 2 grams and Lactobacillus plantarum 1 and 2 grams (adding to water) in controlling the fungal contamination of rainbow trout eggs during the incubation stage and comparing its effects with formalin and It was distilled water. To conduct this study, 18 Californian troughs were used in 6 treatments and three repetitions, and 1850 fertilized rainbow trout eggs were transferred to each trough. After preparing the trays and distributing the eggs, a separate serum was used for each treatment to disinfect the eggs. The duration of complete emptying of each serum was 50 minutes. The results showed that in the treatments containing formalin (10±1) followed by Lactobacillus 2 grams (15.33±3.21), the lowest rate of fungal infection of eggs was observed, which showed a significant difference with other treatments. P>0.05). The highest rate of eye opening (84.67±0.58) and egg opening (81±1) was observed in formalin treatment, which was significantly different from other treatments (P<0.05) https://japb.guilan.ac.ir/article_6853_bda851462ca909f2feba6923ebcffa2f.pdf