University of Guilan Aquatic Physiology and Biotechnology 2345-3966 11 4 2024 03 18 Study of Abramis brama (Berg, 1905) infection with Ligula intestinalis (Linnaeus, 1758) in Siah Darvishan River and survey of them molecular diversity by evaluation of the ribosomal ITS2 region Study of Abramis brama (Berg, 1905) infection with Ligula intestinalis (Linnaeus, 1758) in Siah Darvishan River and survey of them molecular diversity by evaluation of the ribosomal ITS2 region 1 14 7688 10.22124/japb.2023.24804.1501 FA Emad Ahmadiara Assistant Professor in Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran Ali Nikpay Assistant Professor in Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran Journal Article 2023 06 24 Ligula intestinalis (Lineaus1758) is one of the most common parasites of freshwater fish. This study was conducted to survey the possibility of infection of freshwater breams (Abramis brama) caught from Siahdarvishan River and their morphological and molecular characteristics were investigated. 75 breams, after dissection, all parts of their abdominal cavity completely were examined for the presence of the L. intestinalis plerocercoids. Then, the morphometric characteristics of parasites were investigated. In the next step, the definitive identification of the genus and species of parasitic samples was confirmed by identification keys. For molecular analysis, the samples were stored at -20°C. Then, molecular studies were done on 10 plerocercoids obtained from 10 separate fish by analysis of ITS2 gene. From 75 A. brama samples, 18 fish (24%) were infected by L. intestinalis. In this study, the minimum, mean and maximum size of the isolated plerocercoids were 8, 26 and 74cm, respectively. Sequencing of the ITS2 gene of L. intestinalis isolates with GenBank samples showed that these isolates had genetic differences in different hosts and based on the comparison of the ITS2 gene locus, and the presence of host-dependent species in the genus Ligula is probable. Ligula intestinalis (Lineaus1758) is one of the most common parasites of freshwater fish. This study was conducted to survey the possibility of infection of freshwater breams (Abramis brama) caught from Siahdarvishan River and their morphological and molecular characteristics were investigated. 75 breams, after dissection, all parts of their abdominal cavity completely were examined for the presence of the L. intestinalis plerocercoids. Then, the morphometric characteristics of parasites were investigated. In the next step, the definitive identification of the genus and species of parasitic samples was confirmed by identification keys. For molecular analysis, the samples were stored at -20°C. Then, molecular studies were done on 10 plerocercoids obtained from 10 separate fish by analysis of ITS2 gene. From 75 A. brama samples, 18 fish (24%) were infected by L. intestinalis. In this study, the minimum, mean and maximum size of the isolated plerocercoids were 8, 26 and 74cm, respectively. Sequencing of the ITS2 gene of L. intestinalis isolates with GenBank samples showed that these isolates had genetic differences in different hosts and based on the comparison of the ITS2 gene locus, and the presence of host-dependent species in the genus Ligula is probable.

https://japb.guilan.ac.ir/article_7688_f2e6b5251eab4820b207598ed9e6182f.pdf