University of Guilan Aquatic Physiology and Biotechnology 2345-3966 12 1 2024 06 19 Investigating the cytotoxic properties of Chrysaora hysoscella jellyfish venom against human colon cancer cells Investigating the cytotoxic properties of <i>Chrysaora hysoscella</i> jellyfish venom against human colon cancer cells 1 18 7866 10.22124/japb.2024.26396.1523 FA Asghar Mohammadzad Asl Ph.D. Student in Marine Biology, Department of Marine Biology, Faculty of Marine Science and Oceanography, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran Hossein Zolgharnein Professor in Department of Marine Biology, Faculty of Marine Science and Oceanography, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran Soheila Matroodi Assistant Professor in Department of Marine Biology, Faculty of Marine Science and Oceanography, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran Fatemeh Tahoori Assistant Professor in Organic Chemistry, Razi Vaccine and Serum Research Institute, Agricultural Research and Extension Organization (AREEO), Karaj, Iran Zahra Salehi-Najafabadi Assistant Professor in Biotechnology, Razi Vaccine and Serum Research Institute, Agricultural Research and Extension Organization (AREEO), Karaj, Iran Journal Article 2024 01 06 Jellyfish venom contains a variety of compounds including several potential therapeutic properties. The present study investigates the venom of <em>Chrysaora hysoscella</em> jellyfish and analyzes its cytotoxic properties. The fractions of                   <em>C. hysoscella</em> jellyfish venom were purified using HPLC method, and their molecular weight was determined by SDS-PAGE method. The cytotoxic properties of the fractions were evaluated using the MTT method against HCT116 and Caco-2 cancer cell lines as well as RAW 264.7 normal cells. Based on the obtained results, the raw venom of <em>C. hysoscella</em> had 10 fractions with a molecular weight of 15 to 170 kilodaltons, of which fraction 2 had a strong cytotoxic effect against the studied cancer cell lines. The value of IC₅₀ against HCT116 and Caco-2 cells was 63.21 and 64.36 μg/mL, respectively, while against RAW 264.7 cells, it was 122.88 μg/mL. The obtained results revealed the specific cytotoxic property of fraction 2 against colon cancer cells, while its cytotoxic effect on normal cells was significantly less. These results introduce the fraction 2 as a potential anticancer agent for colon cancer. Jellyfish venom contains a variety of compounds including several potential therapeutic properties. The present study investigates the venom of <em>Chrysaora hysoscella</em> jellyfish and analyzes its cytotoxic properties. The fractions of                   <em>C. hysoscella</em> jellyfish venom were purified using HPLC method, and their molecular weight was determined by SDS-PAGE method. The cytotoxic properties of the fractions were evaluated using the MTT method against HCT116 and Caco-2 cancer cell lines as well as RAW 264.7 normal cells. Based on the obtained results, the raw venom of <em>C. hysoscella</em> had 10 fractions with a molecular weight of 15 to 170 kilodaltons, of which fraction 2 had a strong cytotoxic effect against the studied cancer cell lines. The value of IC₅₀ against HCT116 and Caco-2 cells was 63.21 and 64.36 μg/mL, respectively, while against RAW 264.7 cells, it was 122.88 μg/mL. The obtained results revealed the specific cytotoxic property of fraction 2 against colon cancer cells, while its cytotoxic effect on normal cells was significantly less. These results introduce the fraction 2 as a potential anticancer agent for colon cancer. https://japb.guilan.ac.ir/article_7866_a3801782d552f8cc83d0ecc8015aedef.pdf