University of Guilan Aquatic Physiology and Biotechnology 2345-3966 12 1 2024 06 19 Investigating the bioremoval ability of crude oil by bacteria isolated from oil-contaminated soil around Shiraz refinery Investigating the bioremoval ability of crude oil by bacteria isolated from oil-contaminated soil around Shiraz refinery 109 130 7890 10.22124/japb.2023.24454.1496 FA Sara Shokranian M.Sc. in Environmental and Industrial Biotechnology, Department of Marine Biology, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran Morteza Yousefzadi Professor in Department of Biology, Faculty of Sciences, University of Qom, Qom, Iran Narges Amrollahi Biuki Associate Professor in Department of Marine Biology, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran Journal Article 2023 05 06 Today, oil pollution has evolved into a global environmental concern. One of the methods for mitigating this pollution is the use of biological approaches, especially microorganisms, known for their cost-effectiveness and environmental friendliness, rendering them particularly popular among various methods. This study aimed to explore the potential growth of oil-degrading bacteria in oil-contaminated soils, as well as their ability to remove crude oil. Based on bacterial growth rates in the presence of crude oil and hydrocarbon degradation capabilities, two strains, Sludge C and SHA, were selected from among 13 isolates obtained from oil-contaminated soil near the Shiraz refinery. Subsequently, they were assessed for oil degradation using spectrophotometry and gas chromatography methods. Results indicated that Sludge C and SHA possess oil consumption abilities of 64.4% and 84.2%, respectively, within 7 days. Gas chromatography analysis highlighted SHA's remarkable capability in removing short, medium, and long-chain hydrocarbons. Molecular identification confirmed the affiliation of this strain with the genus and species <em>Acinetobacter junii</em>. With its high oil consumption potential, this strain holds promise for efficient utilization in environmental remediation and industry. Today, oil pollution has evolved into a global environmental concern. One of the methods for mitigating this pollution is the use of biological approaches, especially microorganisms, known for their cost-effectiveness and environmental friendliness, rendering them particularly popular among various methods. This study aimed to explore the potential growth of oil-degrading bacteria in oil-contaminated soils, as well as their ability to remove crude oil. Based on bacterial growth rates in the presence of crude oil and hydrocarbon degradation capabilities, two strains, Sludge C and SHA, were selected from among 13 isolates obtained from oil-contaminated soil near the Shiraz refinery. Subsequently, they were assessed for oil degradation using spectrophotometry and gas chromatography methods. Results indicated that Sludge C and SHA possess oil consumption abilities of 64.4% and 84.2%, respectively, within 7 days. Gas chromatography analysis highlighted SHA's remarkable capability in removing short, medium, and long-chain hydrocarbons. Molecular identification confirmed the affiliation of this strain with the genus and species <em>Acinetobacter junii</em>. With its high oil consumption potential, this strain holds promise for efficient utilization in environmental remediation and industry. https://japb.guilan.ac.ir/article_7890_389edd8e26b356269b794955e5998d60.pdf