University of Guilan Aquatic Physiology and Biotechnology 2345-3966 12 1 2024 06 19 Evaluation of the effect of henna plant (Lawsonia inermis) extract compared to copper sulfate (CuSo4.5H2o) on blood, immunity and stress indices and antioxidant activity in liver tissues of Siberian sturgeon (Acipenser baerii) Evaluation of the effect of henna plant (<i>Lawsonia inermis</i>) extract compared to copper sulfate (CuSo4.5H2o) on blood, immunity and stress indices and antioxidant activity in liver tissues of Siberian sturgeon (<i>Acipenser baerii</i>) 131 159 7936 10.22124/japb.2023.24825.1502 FA Ruhollah Barzgar Ph.D. Student in Fisheries, Department of Fisheries, Lahijan Branch, Islamic Azad University, Lahijan, Iran Masoud Farokhrouz Assistant Professor in Department of Fisheries, Lahijan Branch, Islamic Azad University, Lahijan, Iran Hossein Khara Associate Professor in Department of Fisheries, Lahijan Branch, Islamic Azad University, Lahijan, Iran Mohaddeseh Ahmadnezhad Assistant Professor in Inland Water Aquaculture Research Institute, National Fisheries Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Anzali, Iran Alireza Shenavar Masouleh Assistant Professor in International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran Journal Article 2023 06 27 Henna plants (<em>Lawsonia inermis</em>) and copper sulfate (CuSO₄.5H₂O) are used for healing and repairing skin wounds due to their antibiotic and antifungal properties. For this purpose, 500 pieces of Siberian sturgeons with an average weight of 15±1.2g were used. First, the acute toxicity (LC₅₀) and then the maximum permissible concentration (MAC) were determined. To experiment, five treatments with three replications were designed. In treatments 1 to 4, doses equal to henna MAC value and amounts equal to 25%, 50%, and 75% of MAC were used. In treatment 5, copper sulfate at the concentration of 0.07 mg/L was used to compare. Fish were affected by compounds with determined concentrations for 4 days (96 hours). The results showed that henna plant extract performed better than copper sulfate in indicators such as white blood cell count, stress reduction, and liver enzyme activity. Finally, the present study showed that henna plant extract performed better and can be used as a suitable substitute for copper sulfate for disinfection in Siberian sturgeon farms. Also, the results showed that henna plant extract has negative effects on the blood and biochemical indices of Siberian sturgeon. Henna plants (<em>Lawsonia inermis</em>) and copper sulfate (CuSO₄.5H₂O) are used for healing and repairing skin wounds due to their antibiotic and antifungal properties. For this purpose, 500 pieces of Siberian sturgeons with an average weight of 15±1.2g were used. First, the acute toxicity (LC₅₀) and then the maximum permissible concentration (MAC) were determined. To experiment, five treatments with three replications were designed. In treatments 1 to 4, doses equal to henna MAC value and amounts equal to 25%, 50%, and 75% of MAC were used. In treatment 5, copper sulfate at the concentration of 0.07 mg/L was used to compare. Fish were affected by compounds with determined concentrations for 4 days (96 hours). The results showed that henna plant extract performed better than copper sulfate in indicators such as white blood cell count, stress reduction, and liver enzyme activity. Finally, the present study showed that henna plant extract performed better and can be used as a suitable substitute for copper sulfate for disinfection in Siberian sturgeon farms. Also, the results showed that henna plant extract has negative effects on the blood and biochemical indices of Siberian sturgeon. https://japb.guilan.ac.ir/article_7936_1c45300953566b90f3f0618c0479799e.pdf