University of Guilan Aquatic Physiology and Biotechnology 2345-3966 12 2 2024 09 21 Anesthetic effects of peppermint (Mentha piperita) essential oil in comparison with clove (Syzygium aromaticum) essential oil on rainbow trout (Oncorhynchus mykiss) Anesthetic effects of peppermint (<i>Mentha piperita</i>) essential oil in comparison with clove (<i>Syzygium aromaticum</i>) essential oil on rainbow trout (<i>Oncorhynchus mykiss</i>) 115 95 8096 10.22124/japb.2023.25471.1512 FA Edris Rahimi Kia Assistant Professor in Department of Animal Science Research, Center for Research and Education of Agriculture and Natural Resources of Kohgiluyeh and Boir Ahmad Province, of Agricultural Research, Education and Extension Organization, Yasouj, Iran Seyed Hosein Moradian Ph.D. in Fisheries, Shahid Motahari Yasouj Coldwater Fish Genetics and Breeding Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Yasouj, Iran Esmaeil Kazemi Ph.D. in Fisheries, Shahid Motahari Yasouj Coldwater Fish Genetics and Breeding Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Yasouj, Iran Ali Khosravanizadeh Assistant Professor in Department of Aquatic Sciences, Hamoun International Wetland Institute, Research Institute of Zabol, Zabol, Iran Journal Article 2023 09 04 In this research, the anesthetic effects of peppermint essential oil with concentrations of 100, 150, 200, 250 and 300 mg/L and its comparison with concentrations of 20, 30, 40, 60, 80 and 100 mg/L of clove essential oil were evaluated in rainbow trout. 132 fish with an average weight of 23.31±1.27g were used for anesthesia experiments. The induction time of anesthesia using peppermint essential oil varied from 1.69 to 4.81 minutes among different concentrations. In the case of clove essential oil, this time varied from 0.82 to 2.51 minutes in different concentrations. Recovery from complete anesthesia in both anesthetics significantly increase with increasing concentration of the anesthetic. The recovery time from complete anesthesia in the case of peppermint essential oil was from 2.50 to 5.55 minutes and in fish anesthetized with Clove essential oil varied from 2.96 to 3.87. Based on the ideal criteria of induction of anesthesia (less than 3 minutes) and recovery from anesthesia (less than 5 minutes), it is recommended that the most suitable concentration of peppermint essential oil to anesthetize rainbow trout in the tested weight is 200mg/L and the most suitable concentration of clove essential oil is 30mg/L. In this research, the anesthetic effects of peppermint essential oil with concentrations of 100, 150, 200, 250 and 300 mg/L and its comparison with concentrations of 20, 30, 40, 60, 80 and 100 mg/L of clove essential oil were evaluated in rainbow trout. 132 fish with an average weight of 23.31±1.27g were used for anesthesia experiments. The induction time of anesthesia using peppermint essential oil varied from 1.69 to 4.81 minutes among different concentrations. In the case of clove essential oil, this time varied from 0.82 to 2.51 minutes in different concentrations. Recovery from complete anesthesia in both anesthetics significantly increase with increasing concentration of the anesthetic. The recovery time from complete anesthesia in the case of peppermint essential oil was from 2.50 to 5.55 minutes and in fish anesthetized with Clove essential oil varied from 2.96 to 3.87. Based on the ideal criteria of induction of anesthesia (less than 3 minutes) and recovery from anesthesia (less than 5 minutes), it is recommended that the most suitable concentration of peppermint essential oil to anesthetize rainbow trout in the tested weight is 200mg/L and the most suitable concentration of clove essential oil is 30mg/L. https://japb.guilan.ac.ir/article_8096_e180b4ff31a91c5ca8c56f57a50a8a89.pdf