University of GuilanAquatic Physiology and Biotechnology2345-396610220220923Identification and isolation of sprmatogonial stem cells from beluga ( Huso huso) testicular tissueIdentification and isolation of sprmatogonial stem cells from beluga (<i> Huso huso</i>) testicular tissue124590210.22124/japb.2021.20253.1430FAMahsaBorhaniPh.D. Student in Fisheries Science, Department of Fisheries, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University, Noor, IranMohammad RezaKalbassiProfessor in Department of Fisheries, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University, Noor, IranMartinPsenickaAssociate Professor in Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protections of Waters, South Bohemia University, Ceske Budejovice, Czech.BahramFalahatkarProfessor in Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, IranAlexanderCrawfordAssociate Professor in Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, NorwayJournal Article20210729Spermatogonial stem cells (SSCs) are unique cells and able to transmit genetic information to the next generation, therefore, they play an important role in chimeric fish production and preservation of rare species. This study was performed for the first time with the aim of examining morphology of spermatogonial cells through histological method and then isolating them through enzymatic digestion method from beluga (<em>Huso huso</em>) testicular tissue. Histological examination of testicular tissue of one- to three-year-old beluga showed there are two general types of spermatogonial cells, undifferentiated spermatogonia (SSCs) and differentiated spermatogonia. SSCs were often identified by their large size, irregular nuclear envelope, distinct nuclear components and one to two nuclei. Moreover, in one-year-old fish the frequency of SSCs was significantly higher than 2 and 3-year-old beluga (P<0.05). Isolation of testicular tissue cells of one-year-old beluga through enzymatic digestion was indicated that using 0.1% trypsin enzyme with phosphate buffer for 2 to 3 hours at 16-20°C has higher efficiency based on frequency and viability of beluga spermatogonial cells (P<0.05). This study has provided basic information for isolation beluga SSCs for transplantation to produce chimeric fish.Spermatogonial stem cells (SSCs) are unique cells and able to transmit genetic information to the next generation, therefore, they play an important role in chimeric fish production and preservation of rare species. This study was performed for the first time with the aim of examining morphology of spermatogonial cells through histological method and then isolating them through enzymatic digestion method from beluga (<em>Huso huso</em>) testicular tissue. Histological examination of testicular tissue of one- to three-year-old beluga showed there are two general types of spermatogonial cells, undifferentiated spermatogonia (SSCs) and differentiated spermatogonia. SSCs were often identified by their large size, irregular nuclear envelope, distinct nuclear components and one to two nuclei. Moreover, in one-year-old fish the frequency of SSCs was significantly higher than 2 and 3-year-old beluga (P<0.05). Isolation of testicular tissue cells of one-year-old beluga through enzymatic digestion was indicated that using 0.1% trypsin enzyme with phosphate buffer for 2 to 3 hours at 16-20°C has higher efficiency based on frequency and viability of beluga spermatogonial cells (P<0.05). This study has provided basic information for isolation beluga SSCs for transplantation to produce chimeric fish.https://japb.guilan.ac.ir/article_5902_b8f4592ab93c302505790ed5c7ffe303.pdfUniversity of GuilanAquatic Physiology and Biotechnology2345-396610220220923Effects of alga Sargassum angustifolium extract on growth performance, survival rate and body composition of the Asian sea bass ( Lates calcarifer)Effects of alga <i>Sargassum angustifolium</i> extract on growth performance, survival rate and body composition of the Asian sea bass ( <i>Lates calcarifer</i>)2540592510.22124/japb.2021.20331.1432FALalehMosavi DehmordiAssistant Professor in Fisheries Department, Faculty of Natural Resources, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran0000-0002-7779-0739ZahraTaiebzadehM.Sc. Student in Fisheries Engineering, Fisheries Department, Faculty of Natural Resources, Behbahan Khatam Alanbia University of Technology, Behbahan, IranMortezaSoriM.Sc. in Aquaculture Department, General Fisheries of Khuzestan, Abadan, IranJournal Article20210809The aim of this study was to evaluate the effect of macroalga<em> Sargassum angustifolium</em> extract on growth performance, survival and carcass composition of Asian sea bass, <em>Lates calcarifer</em>. For this purpose, 144 pieces of fish with an average weight of 37g were completely randomized with 4 treatments and 3 replications for 56 days, and were fed diets containing 0 (control), 100, 200 and 400mg of alga extract per kg of food. The results of this study showed that the use of alga extract at the rate of 200mg/kg could improve growth and nutrition performance (P<0.05), but the higher concentration of this alga in the diet had opposite effect. The results of carcass biochemical composition analysis also showed that changing the concentration of algae extract in the diet of fish did not have a significant effect on moisture, lipid and protein of this fish (P<0.05). Therefore, the extract of this alga at a concentration of 200mg/kg can be considered as a supplement to growth and nutrition in the diet of Asian sea bassThe aim of this study was to evaluate the effect of macroalga<em> Sargassum angustifolium</em> extract on growth performance, survival and carcass composition of Asian sea bass, <em>Lates calcarifer</em>. For this purpose, 144 pieces of fish with an average weight of 37g were completely randomized with 4 treatments and 3 replications for 56 days, and were fed diets containing 0 (control), 100, 200 and 400mg of alga extract per kg of food. The results of this study showed that the use of alga extract at the rate of 200mg/kg could improve growth and nutrition performance (P<0.05), but the higher concentration of this alga in the diet had opposite effect. The results of carcass biochemical composition analysis also showed that changing the concentration of algae extract in the diet of fish did not have a significant effect on moisture, lipid and protein of this fish (P<0.05). Therefore, the extract of this alga at a concentration of 200mg/kg can be considered as a supplement to growth and nutrition in the diet of Asian sea basshttps://japb.guilan.ac.ir/article_5925_41105c45fc30a3439260793d53e2ea71.pdfUniversity of GuilanAquatic Physiology and Biotechnology2345-396610220220923Effect of density on performance and some hemolymph antioxidant parameters of white-leg shrimp (Litopenaeus vannamei) in a
biofloc systemEffect of density on performance and some hemolymph antioxidant parameters of white-leg shrimp (<i>Litopenaeus vannamei</i>) in a
biofloc system4162592610.22124/japb.2022.21352.1451FAMaryamIraniPh.D. Student in Fisheries, Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, IranHoumanRajabi IslamiAssociate Professor in Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, IranMahmoudNafisi BahabadiAssociate Professor in Department of Fisheries, Faculty of Agriculture and Natural Resources, Persian Gulf University, Bushehr, IranSeye PezhmanHosseini ShekarabiAssistant Professor in Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, IranJournal Article20211221The present study was designed to investigate the effect of density on some performance and activity of biochemical parameters of white-leg shrimp (Litopenaeus vannamei). Twenty-day-old larvae (PL30) were distributed at densities of 100, 200, 300 and 400 No. m-2 in twelve polyethylene tanks (working volume of 200 L) containing bioflocks and fed ad libitum using artificial feed. Each triplicate tank was assigned to one treatment. At the end of experiment, performance indicators, antioxidant enzymes activities and some of the stress parameters were determined. The results showed that an increase in the shrimp density under the biofloc system had a significant effect on the growth performance and hemolymph biochemical properties of white-leg shrimp and declined the final weight, daily growth rate and feeding efficiency ratio. However, the amount of biomass increased with increasing density and reached to more than 1.7 folds higher than that in under the density of 100 No. m-2. However, the antioxidant defense parameters including the activity level of superoxide dismutase, catalase and glutathione peroxidase enhanced with increasing the shrimp density in the biofloc system. Cortisol and glucose levels of the hemolymph also showed a decreasing trend with increasing density to 300 No. m-2,The present study was designed to investigate the effect of density on some performance and activity of biochemical parameters of white-leg shrimp (Litopenaeus vannamei). Twenty-day-old larvae (PL30) were distributed at densities of 100, 200, 300 and 400 No. m-2 in twelve polyethylene tanks (working volume of 200 L) containing bioflocks and fed ad libitum using artificial feed. Each triplicate tank was assigned to one treatment. At the end of experiment, performance indicators, antioxidant enzymes activities and some of the stress parameters were determined. The results showed that an increase in the shrimp density under the biofloc system had a significant effect on the growth performance and hemolymph biochemical properties of white-leg shrimp and declined the final weight, daily growth rate and feeding efficiency ratio. However, the amount of biomass increased with increasing density and reached to more than 1.7 folds higher than that in under the density of 100 No. m-2. However, the antioxidant defense parameters including the activity level of superoxide dismutase, catalase and glutathione peroxidase enhanced with increasing the shrimp density in the biofloc system. Cortisol and glucose levels of the hemolymph also showed a decreasing trend with increasing density to 300 No. m-2,https://japb.guilan.ac.ir/article_5926_bedf03ebdf9c8c4f1fdc6f70e643daee.pdfUniversity of GuilanAquatic Physiology and Biotechnology2345-396610220220923Effects of different dietary levels of Azomite on muscle antioxidant defense, survival rate and resistance to Aeromonas hydrophila in juvenile common carp (Cyprinus carpio )Effects of different dietary levels of Azomite on muscle antioxidant defense, survival rate and resistance to <i>Aeromonas hydrophila </i> in juvenile common carp (<i>Cyprinus carpio</i> )6384592410.22124/japb.2021.20566.1436FAMonaTafaniM.Sc. in Fisheries, Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, IranNasimZangueeAssistant Professor in Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, IranAmir ParvizSalatiProfessor in Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, IranMohammadZakeriProfessor in Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, IranSeyed MohammadMousaviProfessor in Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, IranMohammad JavadImaniM.Sc. in Fisheries, Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, IranJournal Article20210912The aim of this study was to evaluate the effects of Azomite on muscle antioxidant defense, survival rate and resistance to <em>Aeromonas hydrophila</em> experimental infection in juvenile common carp. For this purpose, a total of 180 fish, with initial average weight of 20.05±4.15g, were randomly divided in 4 treatments and fed 4 different diets including control group (without Azomite), treatment 1 (2g/kg Azomite), treatment 2 (4g/kg Azomite) and treatment 3 (8g/kg Azomite) for a period of 8 weeks. The results showed that the muscle antioxidant defense was not significantly affected by Azomite consumption (P>0.05). However, the highest activity of catalase and glutathione peroxidase was observed in treatment 3. Oral administration of Azomite had no significant effect on the fish survival rate during the period of experiment (P>0.05). But the resistance to <em>A. hydrophila</em> was significantly increased and the highest resistance (the lowest mortality rate) was related to treatment 2, which showed a significant difference with the control group (P<0.05). According to results, it can be suggested that adding 4g/kg of Azomite to common carp diet can increase resistance to <em>A. hydrophila</em> infection without having any negative effect on muscle antioxidant defense system.The aim of this study was to evaluate the effects of Azomite on muscle antioxidant defense, survival rate and resistance to <em>Aeromonas hydrophila</em> experimental infection in juvenile common carp. For this purpose, a total of 180 fish, with initial average weight of 20.05±4.15g, were randomly divided in 4 treatments and fed 4 different diets including control group (without Azomite), treatment 1 (2g/kg Azomite), treatment 2 (4g/kg Azomite) and treatment 3 (8g/kg Azomite) for a period of 8 weeks. The results showed that the muscle antioxidant defense was not significantly affected by Azomite consumption (P>0.05). However, the highest activity of catalase and glutathione peroxidase was observed in treatment 3. Oral administration of Azomite had no significant effect on the fish survival rate during the period of experiment (P>0.05). But the resistance to <em>A. hydrophila</em> was significantly increased and the highest resistance (the lowest mortality rate) was related to treatment 2, which showed a significant difference with the control group (P<0.05). According to results, it can be suggested that adding 4g/kg of Azomite to common carp diet can increase resistance to <em>A. hydrophila</em> infection without having any negative effect on muscle antioxidant defense system.https://japb.guilan.ac.ir/article_5924_18050499fea40648c414f067e37bf80c.pdfUniversity of GuilanAquatic Physiology and Biotechnology2345-396610220220923Effects of dietary procell alone and in combination with Lactobacillus plantarum and Lactobacillus pentosus on serum biochemical parameters and some oxidative enzymes of rainbow trout (Oncorhynchus mykiss)Effects of dietary procell alone and in combination with <i>Lactobacillus plantarum</i> and <i>Lactobacillus pentosus</i> on serum biochemical parameters and some oxidative enzymes of rainbow trout (<i>Oncorhynchus mykiss</i>)85102592910.22124/japb.2021.20521.1435FATakavarMohammadianAssociated Professor in Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranMember of Excellence Center of Warm Water Fish Health, Shahid Chamran University of Ahvaz, Ahvaz, IranAbdolhosseinJangaran Nejad KahediAssistant Professor in Department of Basic Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, IranSeyedeh ParvaMousaviDoctor of Veterinary Medicine, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranMohammad RezaTabandehAssociated Professor in Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranMember of Excellence Center of Warm Water Fish Health, Shahid Chamran University of Ahvaz, Ahvaz, IranHodaLababianDoctor of Veterinary Medicine, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranSadeghRobat KarimiDoctor of Veterinary Medicine, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranJournal Article20210905Intensive and commercial aquaculture need to improve the fish welfare and probiotics are considered as a suitable candidate to achieve this goal. In this study, the effect of procell synbiotic supplement is evaluated as alone and in combination with endogenous bacteria on biochemical parameters and some oxidative enzymes in rainbow trout serum. For this purpose, 300 rainbow trout<strong> </strong>fingerlings with average weight of 15g were randomly divided into five groups with three replicates, which were fed for 12 weeks with normal diet and without any additives (control), procell synbiotic at 1%, procell synbiotic 1% and <em>Lactobacillus plantarum</em> at 10<sup>8</sup>CFU/mg, procell symbiotic 1% and <em>Lactobacillus pentosus</em> at 10<sup>8</sup>CFU/mg and procell synbiotic 1% and <em>L.</em> <em>plantarum</em> and <em>L.</em> <em>pentosus</em> at 10<sup>8</sup>CFU/mg, respectively. Sampling was performed on days 0 and 60. The results showed that the use of diets containing procell+<em>L. plantarum</em>+<em>L. pentosus</em> is able to improve the biochemical, antioxidant and liver enzymes in rainbow trout fingerling serum.Intensive and commercial aquaculture need to improve the fish welfare and probiotics are considered as a suitable candidate to achieve this goal. In this study, the effect of procell synbiotic supplement is evaluated as alone and in combination with endogenous bacteria on biochemical parameters and some oxidative enzymes in rainbow trout serum. For this purpose, 300 rainbow trout<strong> </strong>fingerlings with average weight of 15g were randomly divided into five groups with three replicates, which were fed for 12 weeks with normal diet and without any additives (control), procell synbiotic at 1%, procell synbiotic 1% and <em>Lactobacillus plantarum</em> at 10<sup>8</sup>CFU/mg, procell symbiotic 1% and <em>Lactobacillus pentosus</em> at 10<sup>8</sup>CFU/mg and procell synbiotic 1% and <em>L.</em> <em>plantarum</em> and <em>L.</em> <em>pentosus</em> at 10<sup>8</sup>CFU/mg, respectively. Sampling was performed on days 0 and 60. The results showed that the use of diets containing procell+<em>L. plantarum</em>+<em>L. pentosus</em> is able to improve the biochemical, antioxidant and liver enzymes in rainbow trout fingerling serum.https://japb.guilan.ac.ir/article_5929_661a57580f262c597db6a6d616e014ed.pdfUniversity of GuilanAquatic Physiology and Biotechnology2345-396610220220923Antioxidant properties of fucoidan extracted from brown alga Sargassum (Sargassum tenerrimum) and its ability to inhibit vannamei shrimp polyphenol oxidase enzymeAntioxidant properties of fucoidan extracted from brown alga Sargassum (<i>Sargassum tenerrimum</i>) and its ability to inhibit vannamei shrimp polyphenol oxidase enzyme103119594310.22124/japb.2021.20417.1434FASaeidGorgijM.Sc. in Fisheries, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, IranSalimSharifianAssistant Professor in Fisheries Department, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran0000-0003-2669-8091MehranLoghaminAssociate Professor in Marine Biology Department, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, IranJournal Article20210820Fucoidan is one of the most important polysaccharides produced by brown seaweeds. In the present study, fucoidan was extracted from brown seaweed <em>Sargassum tenerrimum</em> and its properties include the extraction yield, carbohydrates, protein, sulfate, uronic acid content, antioxidant properties (DPPH free-radical scavenging and ferric reducing power) and its ability to inhibit polyphenol oxidase enzyme from vannamei shrimp (<em>Litopenaeus vannamei</em>) were assayed at concentrations of 0.5, 1 and 2mg/mL. The amount of the extraction yield, carbohydrate, protein, sulfates and uronic acid of the extracted fucoidan were 7.23, 36.51, 5.64, 11.57 and 9.22%, respectively. The rate of DPPH scavenging in fucoidan varied from 35.94 to 63.94% depending on the concentration (P<0.05). The rate of iron reduction varied from 0.23 in fucoidan at a concentration of 0.5mg/mL to 0.74 in treatment of 2mg/mL fucoidan. The highest inhibition of vannamei shrimp polyphenol oxidase enzyme was obtained in fucoidan with a concentration of 2mg/mL and equal to 64.72%. The results of this study showed that brown seaweed <em>Sargassum</em> <em>tenerrimum</em> is a rich source of fucoidan with significant antioxidant properties and polysaccharides extracted from this alga can be a natural and healthy inhibitor of polyphenol oxidase.Fucoidan is one of the most important polysaccharides produced by brown seaweeds. In the present study, fucoidan was extracted from brown seaweed <em>Sargassum tenerrimum</em> and its properties include the extraction yield, carbohydrates, protein, sulfate, uronic acid content, antioxidant properties (DPPH free-radical scavenging and ferric reducing power) and its ability to inhibit polyphenol oxidase enzyme from vannamei shrimp (<em>Litopenaeus vannamei</em>) were assayed at concentrations of 0.5, 1 and 2mg/mL. The amount of the extraction yield, carbohydrate, protein, sulfates and uronic acid of the extracted fucoidan were 7.23, 36.51, 5.64, 11.57 and 9.22%, respectively. The rate of DPPH scavenging in fucoidan varied from 35.94 to 63.94% depending on the concentration (P<0.05). The rate of iron reduction varied from 0.23 in fucoidan at a concentration of 0.5mg/mL to 0.74 in treatment of 2mg/mL fucoidan. The highest inhibition of vannamei shrimp polyphenol oxidase enzyme was obtained in fucoidan with a concentration of 2mg/mL and equal to 64.72%. The results of this study showed that brown seaweed <em>Sargassum</em> <em>tenerrimum</em> is a rich source of fucoidan with significant antioxidant properties and polysaccharides extracted from this alga can be a natural and healthy inhibitor of polyphenol oxidase.https://japb.guilan.ac.ir/article_5943_366efb7dda0f2157a2d133f23aa709c7.pdfUniversity of GuilanAquatic Physiology and Biotechnology2345-396610220220923Comparison of gene expression of mantle tissue in Pinctada persica between warm and cold seasonsComparison of gene expression of mantle tissue in <i>Pinctada persica</i> between warm and cold seasons121139595010.22124/japb.2021.20297.1431FAFatemehParviziPh.D. in Aquaculture, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, IranAbolfazlNajiAssociated Professor in Department of Fisheries, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, IranArashAkbarzadehAssociated Professor in Department of Fisheries, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, IranAhmadFarhadiAssistant Professor in Department of Natural Resources and Environmental Engineering, Faculty of Agricultural Sciences, Shiraz University, Shiraz, IranMohammad SharifRanjbarAssociated Professor in Department of Marine Biology, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran.Associated Professor in Persian Gulf Biotechnology Park, Qeshm Island, Iran0000-0002-1093-2392Journal Article20210804Regarding the significant effect of temperature on biomineralization process, the study of the seasonal changes impacts on the functions and patterns of gene expression in mantle tissue as an important organ responsible for biomineralization in pearl oysters, is necessary. In this study, the gene expression patterns of 9 biomineralization genes (<em>ASP</em>, <em>KRMP</em>, <em>SHEM5</em>, <em>PRISM</em>, <em>PEARL</em>, <em>CHIT</em>, <em>PIF</em>, <em>MRNP34</em>, <em>NACREIN</em>) were investigated in the mantle tissue of <em>Pinctada persica</em> between warm and cold seasons. The cDNA was constructed using reverse transcriptase enzyme and then the expression of genes were quantified in qPCR. The results showed difference in seasonal pattern. Three genes of prismatic layer (<em>ASP</em>, <em>KRMP and SHEM5</em>) had high expression in cold season and <em>PIF</em> which involved in nacre layer formation, showed high level of expression in warm season. It is likely, the biomineralization rate reduce in higher temperature and the prismatic related genes play more important role in response to seasonal changes. This study has provided valuable information about mantle tissue traits of <em>P. persica</em> and influence of environmental factors in molecular mechanisms involved in the formation of shell and pearl.Regarding the significant effect of temperature on biomineralization process, the study of the seasonal changes impacts on the functions and patterns of gene expression in mantle tissue as an important organ responsible for biomineralization in pearl oysters, is necessary. In this study, the gene expression patterns of 9 biomineralization genes (<em>ASP</em>, <em>KRMP</em>, <em>SHEM5</em>, <em>PRISM</em>, <em>PEARL</em>, <em>CHIT</em>, <em>PIF</em>, <em>MRNP34</em>, <em>NACREIN</em>) were investigated in the mantle tissue of <em>Pinctada persica</em> between warm and cold seasons. The cDNA was constructed using reverse transcriptase enzyme and then the expression of genes were quantified in qPCR. The results showed difference in seasonal pattern. Three genes of prismatic layer (<em>ASP</em>, <em>KRMP and SHEM5</em>) had high expression in cold season and <em>PIF</em> which involved in nacre layer formation, showed high level of expression in warm season. It is likely, the biomineralization rate reduce in higher temperature and the prismatic related genes play more important role in response to seasonal changes. This study has provided valuable information about mantle tissue traits of <em>P. persica</em> and influence of environmental factors in molecular mechanisms involved in the formation of shell and pearl.https://japb.guilan.ac.ir/article_5950_db1a6de9b6e062e813bc2f69f4a059b4.pdf