University of Guilan Aquatic Physiology and Biotechnology 2345-3966 10 3 2022 12 21 Evaluation of antibacterial and anti-biofilm effects of carvacrol and eugenol on bacteria isolated from the Persian Gulf waters Evaluation of antibacterial and anti-biofilm effects of carvacrol and eugenol on bacteria isolated from the Persian Gulf waters 1 22 6107 10.22124/japb.2021.20053.1428 FA Fatemeh Sedaghat Ph.D. Student in Marine Biology, Department of Marine Biology, Faculty of Marine Sciences and Technology, University of Hormozgan, Bandar Abbas, Iran Morteza Yousefzadi Professor in Department of Biology, Faculty of Sciences, University of Qom, Qom, Iran Arash Ghaderi Associated Professor in Department of Chemistry, Faculty of Sciences, University of Hormozgan, Bandar Abbas, Iran Fatemeh Shayesteh Assistant Professor in Department of Fisheries Science, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran Journal Article 2021 07 05 The formation of microbial biofilms is one of the key steps in marine fouling. Therefore, the development of anti-biofilm strategies, especially through research on environmentally friendly antifouling materials, can help control biofouling. The present study was performed to evaluate the antibacterial and anti-biofilm activity of carvacrol and eugenol against marine bacteria isolated from Persian Gulf waters. In disk diffusion method, Vibrio sp. (KM1) bacteria showed more sensitivity against carvacrol and eugenol with a growth inhibition zone diameter of 17±0.3 and 15±0.5mm, respectively. Minimum inhibitory concentration (62.5µg/mL) and minimum bactericidal concentration (125µg/mL) were also obtained with carvacrol against KM1. In this study, the inhibitory effect of carvacrol and eugenol on biofilm formation by Pseudoalteromonas sp. (PH18) using tissue culture plate method (TCP) was investigated and 4MIC concentration showed the highest biofilm inhibition (carvacrol 90% and eugenol 87%). Based on the results, carvacrol and eugenol can be used as an alternative in antifouling coatings due to their appropriate anti-biofilm activity against marine bacteria. The formation of microbial biofilms is one of the key steps in marine fouling. Therefore, the development of anti-biofilm strategies, especially through research on environmentally friendly antifouling materials, can help control biofouling. The present study was performed to evaluate the antibacterial and anti-biofilm activity of carvacrol and eugenol against marine bacteria isolated from Persian Gulf waters. In disk diffusion method, Vibrio sp. (KM1) bacteria showed more sensitivity against carvacrol and eugenol with a growth inhibition zone diameter of 17±0.3 and 15±0.5mm, respectively. Minimum inhibitory concentration (62.5µg/mL) and minimum bactericidal concentration (125µg/mL) were also obtained with carvacrol against KM1. In this study, the inhibitory effect of carvacrol and eugenol on biofilm formation by Pseudoalteromonas sp. (PH18) using tissue culture plate method (TCP) was investigated and 4MIC concentration showed the highest biofilm inhibition (carvacrol 90% and eugenol 87%). Based on the results, carvacrol and eugenol can be used as an alternative in antifouling coatings due to their appropriate anti-biofilm activity against marine bacteria.

https://japb.guilan.ac.ir/article_6107_ac5dbf25981efa4640cf9a747b6c6229.pdf

University of Guilan Aquatic Physiology and Biotechnology 2345-3966 10 3 2022 12 21 Interspecific gynogenesis induction in Siberian sturgeon (Acipenser baerii ) using heterologous sperm Interspecific gynogenesis induction in Siberian sturgeon (Acipenser baerii ) using heterologous sperm 23 48 6108 10.22124/japb.2021.20693.1439 FA Mohammad Hassanzadeh Saber Scientific Member in Genetics and Biotechnology Department, International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Iran Shahrouz Baradaran Noveiri Assistant Professor in Genetics and Biotechnology Department, International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Iran Mohammad Pourkazemi Professor in Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran Mahtab Yarmohammadi - Associate Professor in Genetics and Biotechnology Department, International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Iran Hooshang Yeganeh Raste Kenari M.Sc. in Fisheries, Aquaculture Department, International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Iran Jalil Jalilpoor M.Sc. in Fisheries, Health and Disease Department, International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Iran Journal Article 2021 10 03 Siberian sturgeon (Acipenser baerii) grows rapidly in breeding ponds. Gynogenesis is a suitable technique for producing sturgeon female sex. The aim of this study was to establish female sex in Siberian sturgeon using induction of gynogenesis. The sperm of Persian sturgeon (Acipenser persicus) was genetically inactivated by UV radiation at a dose of 8514 J/m2. In this case, sperm with 40% motility were combined with Siberian sturgeon oocytes and in 2°C cold shock for 30 minutes, 10 minutes after activity, gynogenic progeny were created. Fertilization rate was 44.5% (control hybrid 62%) and hatching rate was 19.8% (control hybrid 40.5%). Purely maternal heritability in gynogeneic progeny was confirmed using microsatellite markers. By achieving the biotechnique of sperm production with inactivated DNA of Persian sturgeon, it is possible to prevent the risk of extinction and protect other sturgeon by inducing gynogenesis. Siberian sturgeon (Acipenser baerii) grows rapidly in breeding ponds. Gynogenesis is a suitable technique for producing sturgeon female sex. The aim of this study was to establish female sex in Siberian sturgeon using induction of gynogenesis. The sperm of Persian sturgeon (Acipenser persicus) was genetically inactivated by UV radiation at a dose of 8514 J/m2. In this case, sperm with 40% motility were combined with Siberian sturgeon oocytes and in 2°C cold shock for 30 minutes, 10 minutes after activity, gynogenic progeny were created. Fertilization rate was 44.5% (control hybrid 62%) and hatching rate was 19.8% (control hybrid 40.5%). Purely maternal heritability in gynogeneic progeny was confirmed using microsatellite markers. By achieving the biotechnique of sperm production with inactivated DNA of Persian sturgeon, it is possible to prevent the risk of extinction and protect other sturgeon by inducing gynogenesis.

https://japb.guilan.ac.ir/article_6108_e5696c7702fbc1e748cee8293ea8b363.pdf

University of Guilan Aquatic Physiology and Biotechnology 2345-3966 10 3 2022 12 21 The effect of short-term nutrition of turmeric (Curcuma langa ) and black pepper (Piper nigrum ) combination on blood parameters, immunity and liver enzymes in rainbow trout (Oncorhynchus mykiss ) The effect of short-term nutrition of turmeric (Curcuma langa ) and black pepper (Piper nigrum ) combination on blood parameters, immunity and liver enzymes in rainbow trout (Oncorhynchus mykiss ) 49 78 6113 10.22124/japb.2022.20710.1437 FA SeyedehMitra Aghili Ph.D. in Reproduction and Breeding of Aquatic Animals, Department of Fisheries, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran Farid Firouzbakhsh Professor in Department of Fisheries, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran Sara Haghparast Assistant Professor in Department of Fisheries, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran Ayoub Farhadi Associate Professor in Department of Animal Science, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran Journal Article 2021 09 28 The aim of this study was to evaluate the effect of short-term feeding of turmeric (Curcuma langa) and black pepper (Piper nigrum) on blood and immune parameters and liver enzymes of rainbow trout (Oncorhynchus mykiss) during 30 days. A total of 450 fish with an average weight of 24.95±0.70g were selected and in ten experimental groups, fed with diet containing turmeric (C) and black pepper (P) in a combined ratio (R) of 30, 50 and 70% at levels (L) of 0 (control), 0.25, 0.5 and 1%. Parameters with independent effect including AST at the level of 0.25%, lysozyme and complement at the level of 0.5% and RBC and hematocrit at the level of 1% had the highest levels. The highest amount of hemoglobin, total protein and the lowest amount of triglyceride and cholesterol were reported in the higher ratio of black pepper to turmeric (30%C + 70%P) at the level of 1% of plant supplement. The highest amount of globulin and total immunoglobulin was observed at the level of 0.25% of the diet and the combined ratio of 70%C + 30%P. Improvement of these parameters can be due to the positive effect of turmeric and black pepper combination on the immunity of rainbow trout. Therefore, it can be suggested that the 1% herbal supplements be used in the diet of rainbow trout. The aim of this study was to evaluate the effect of short-term feeding of turmeric (Curcuma langa) and black pepper (Piper nigrum) on blood and immune parameters and liver enzymes of rainbow trout (Oncorhynchus mykiss) during 30 days. A total of 450 fish with an average weight of 24.95±0.70g were selected and in ten experimental groups, fed with diet containing turmeric (C) and black pepper (P) in a combined ratio (R) of 30, 50 and 70% at levels (L) of 0 (control), 0.25, 0.5 and 1%. Parameters with independent effect including AST at the level of 0.25%, lysozyme and complement at the level of 0.5% and RBC and hematocrit at the level of 1% had the highest levels. The highest amount of hemoglobin, total protein and the lowest amount of triglyceride and cholesterol were reported in the higher ratio of black pepper to turmeric (30%C + 70%P) at the level of 1% of plant supplement. The highest amount of globulin and total immunoglobulin was observed at the level of 0.25% of the diet and the combined ratio of 70%C + 30%P. Improvement of these parameters can be due to the positive effect of turmeric and black pepper combination on the immunity of rainbow trout. Therefore, it can be suggested that the 1% herbal supplements be used in the diet of rainbow trout.

https://japb.guilan.ac.ir/article_6113_8c5856c6dad4b781e3d2e2fb596a66a5.pdf

University of Guilan Aquatic Physiology and Biotechnology 2345-3966 10 3 2022 12 21 Comparison of the effect of copper oxide and iron oxide nanoparticles on oxidative stress in Nannochloropsis oculata Comparison of the effect of copper oxide and iron oxide nanoparticles on oxidative stress in Nannochloropsis oculata 79 94 6114 10.22124/japb.2022.20717.1438 FA Nasrin Fazelian Ph.D. in Plant Physiology, Department of Plant Biology, Faculty of Natural Sciences, Tabriz University, Tabriz, Iran Morteza Yousefzadi Professor in Department of Biology, Faculty of Science, University of Qom, Qom, Iran Journal Article 2021 09 28 In this study, the effects of various concentrations (5, 10, 50, 100 and 200mg/L) of copper oxide (CuO) and iron oxide (Fe2O3) nanoparticles (NPs) on growth, lipid peroxidation and activity of catalase and ascorbate peroxidase enzymes were investigated. The results showed that concentrations of more than 5mg/L of CuO-NPs and more than 10mg/L of Fe2O3-NPs reduced the growth of Nannochloropsis oculata. The content of aldehydes in response to some of concentrations of both metal oxide nanoparticles significantly increased compared to control (P<0.05(. However, the increase of aldehydes in response to CuO-NPs was higher than Fe2O3-NPs. The activity of ascorbate peroxidase increased significantly in response to both nanoparticles, but the effect of CuO-NPs on the activity of this enzyme was higher than that of Fe2O3-NPs. Catalase activity showed a significant increase in response to CuO-NPs, but Fe2O3-NPs decreased the activity of this enzyme. The results showed that the toxicity of CuO-NPs in N. oculata was much more severe than Fe2O3-NPs. In this study, the effects of various concentrations (5, 10, 50, 100 and 200mg/L) of copper oxide (CuO) and iron oxide (Fe2O3) nanoparticles (NPs) on growth, lipid peroxidation and activity of catalase and ascorbate peroxidase enzymes were investigated. The results showed that concentrations of more than 5mg/L of CuO-NPs and more than 10mg/L of Fe2O3-NPs reduced the growth of Nannochloropsis oculata. The content of aldehydes in response to some of concentrations of both metal oxide nanoparticles significantly increased compared to control (P<0.05(. However, the increase of aldehydes in response to CuO-NPs was higher than Fe2O3-NPs. The activity of ascorbate peroxidase increased significantly in response to both nanoparticles, but the effect of CuO-NPs on the activity of this enzyme was higher than that of Fe2O3-NPs. Catalase activity showed a significant increase in response to CuO-NPs, but Fe2O3-NPs decreased the activity of this enzyme. The results showed that the toxicity of CuO-NPs in N. oculata was much more severe than Fe2O3-NPs.

https://japb.guilan.ac.ir/article_6114_70f0c38c6e19e87819ab4b2b06b7108a.pdf

University of Guilan Aquatic Physiology and Biotechnology 2345-3966 10 3 2022 12 21 Effect of Sargassum ilicifolium alcoholic extract on growth performance, blood parameters and digestive enzymatic activities in fingerlimg gold fish ( Carassius auratus) Effect of Sargassum ilicifolium alcoholic extract on growth performance, blood parameters and digestive enzymatic activities in fingerlimg gold fish ( Carassius auratus) 95 117 6118 10.22124/japb.2022.20883.1442 FA Reza Nahavandi Assistant Professor in Biotechnology Department, Animal Science Research Institute of Iran (ASRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran Ali Sadeghi Ph.D. in Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran Meysam Sabzeh M.Sc. in Fisheries, Azad Shahr Branch, Islamic Azad University, Gorgan, Iran Sajjad Pourmozaffar Assistant Professor in Aquaculture Department, , Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Lengeh, Iran Saeid Tamadoni Jahromi Associate Professor in Biotechnology Department, Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas, Iran Mohammad Khalil Pazir Assistant Professor in Health and Diseases of Aquatic Animals Department, Iran Shrimp Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Boushehr, Iran Keyvan Ejlali Assistant Professor in Ecology Department, Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas, Iran Journal Article 2021 10 21 This experiment was conducted to evaluate the effect of different levels of supplementation of Sargassum ilicifolium on the growth performance, blood parameters and digestive enzymatic activities of goldfish (Carassius auratus) for 60 days. Goldfish (with an average initial weight of 5.25±0.45g) were divided into four experimental treatments with three replicates (30 fish/replicate) inluding control treatment (without algae extract) and three treatments supplemented with Sargassum extracts 5, 10 and 15g/kg diet. The results showed that the highest final weight, weight gain, specific growth rate and condition factor were observed in fish fed with 15g/kg S. ilicifolium extract. Hemoglobin and MCH in fish fed with diets containing S. ilicifolium extracts showed significant difference compared to control group. The levels of triglyceride and cholesterol in fish fed with control diet were significantly higher than that of fed diets containing S. ilicifolium extracts (P<0.05). The highest proteases, amylase and lipase activities were recorded in Sargassum extract at 15g/kg diet. The results of this study showed that goldfish fed the diet containing 15g/kg S. ilicifolium had better growth performance and activity of digestive enzymes compared to other groups. Therefor the administration of 15g/kg of S. ilicifolium extract is recommended to use in goldfish diet. This experiment was conducted to evaluate the effect of different levels of supplementation of Sargassum ilicifolium on the growth performance, blood parameters and digestive enzymatic activities of goldfish (Carassius auratus) for 60 days. Goldfish (with an average initial weight of 5.25±0.45g) were divided into four experimental treatments with three replicates (30 fish/replicate) inluding control treatment (without algae extract) and three treatments supplemented with Sargassum extracts 5, 10 and 15g/kg diet. The results showed that the highest final weight, weight gain, specific growth rate and condition factor were observed in fish fed with 15g/kg S. ilicifolium extract. Hemoglobin and MCH in fish fed with diets containing S. ilicifolium extracts showed significant difference compared to control group. The levels of triglyceride and cholesterol in fish fed with control diet were significantly higher than that of fed diets containing S. ilicifolium extracts (P<0.05). The highest proteases, amylase and lipase activities were recorded in Sargassum extract at 15g/kg diet. The results of this study showed that goldfish fed the diet containing 15g/kg S. ilicifolium had better growth performance and activity of digestive enzymes compared to other groups. Therefor the administration of 15g/kg of S. ilicifolium extract is recommended to use in goldfish diet.

https://japb.guilan.ac.ir/article_6118_c0675e02e5adb32fcc6fdb11da72adb0.pdf

University of Guilan Aquatic Physiology and Biotechnology 2345-3966 10 3 2022 12 21 Increased tolerance of Mexican lime (Citrus aurantifolia Swingle) seedlings to salinity stress when inoculated with endophytes spergillus nigerA and Bacillus aquimaris extracted from seaweed Increased tolerance of Mexican lime (Citrus aurantifolia Swingle) seedlings to salinity stress when inoculated with endophytes spergillus nigerA and Bacillus aquimaris extracted from seaweed 119 147 6131 10.22124/japb.2022.20908.1444 FA Leila Baghazadeh Ph.D. in Biotechnology and Molecular Genetics of Horticultural Products, Hormozgan University, Bandar Abbas, Iran Davood Samsampour Associate Professor in Horticultural Science Department, Agriculture and Natural Resources College, University of Hormozgan, Bandar Abbas, Iran Abdoolnabi Bagheri Assistant Professor in Plant Protection Research Department, Hormozgan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas, Iran Jelveh Sohrabipour Assistant Professor in Hormozgan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas, Iran Journal Article 2021 10 24 Endophytes play an important role in protecting plants. In this study, endophytic combination of Aspergillus niger and Bacillus aquimaris OD14 was used to improve tolerance of Mexican lime seedlings to salinity stress. Three months after inoculation, salinity stress of 0, 2000, 4000 and 6000 μs/cm was applied. After 8 weeks, morphological, biochemical and elements adsorption traits of seedlings were evaluated. The experiment was carried out as factorial in a completely randomized design with three replications. The results showed that in 6000 μs/cm water salinity, endophytic compound increased stem length (103.64%), trunk diameter (103.04%), number of leaves (444.41%) and root length and width (232.02% and 41.63% respectively). Endophyte increased 76.29% of SPAD chlorophyll content compared to control. Also, the uptake of root potassium (34.95%) and shoot calcium (115.15%) increased and the uptake of sodium elements was decreased (29.49%) compared to control. Endophytic combination could significantly reduce ion leakage (65.57%) and increase proline content (69.3%) in inoculated seedlings compared to control. In general, marine endophytes can be used as a suitable option to increase plant tolerance to salinity. Endophytes play an important role in protecting plants. In this study, endophytic combination of Aspergillus niger and Bacillus aquimaris OD14 was used to improve tolerance of Mexican lime seedlings to salinity stress. Three months after inoculation, salinity stress of 0, 2000, 4000 and 6000 μs/cm was applied. After 8 weeks, morphological, biochemical and elements adsorption traits of seedlings were evaluated. The experiment was carried out as factorial in a completely randomized design with three replications. The results showed that in 6000 μs/cm water salinity, endophytic compound increased stem length (103.64%), trunk diameter (103.04%), number of leaves (444.41%) and root length and width (232.02% and 41.63% respectively). Endophyte increased 76.29% of SPAD chlorophyll content compared to control. Also, the uptake of root potassium (34.95%) and shoot calcium (115.15%) increased and the uptake of sodium elements was decreased (29.49%) compared to control. Endophytic combination could significantly reduce ion leakage (65.57%) and increase proline content (69.3%) in inoculated seedlings compared to control. In general, marine endophytes can be used as a suitable option to increase plant tolerance to salinity.

https://japb.guilan.ac.ir/article_6131_fb8f4521328c1d7a721e0594acd427c9.pdf