تولید پروتئین هیدرولیز شده از ماهی کیلکا با روش هیدرولیز آنزیمی و سنجش خواص زیست‌فعالی آن

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناس ارشد فرآوری محصولات شیلاتی، گروه فرآوری محصولات شیلاتی، دانشکده علوم دریایی، دانشگاه تربیت مدرس، نور، ایران

2 استادیار گروه فرآوری محصولات شیلاتی، دانشکده علوم دریایی، دانشگاه تربیت مدرس، نور، ایران

3 دانشیار گروه پلیمرهای زیست‌سازگار، پژوهشگاه پلیمر و پتروشیمی ایران، تهران، ایران

چکیده

در مطالعه حاضر، ابتدا با استفاده از آنزیم آلکالاز از ماهی کیلکای معمولی (Clupeonella cultriventris)­ پروتئین هیدرولیز شده (FPH) به دست آمد. سپس فعالیت آنتی‌اکسیدانی پروتئین هیدرولیز شده ماهی  به وسیله آزمون­های قدرت مهارکنندگی رادیکال‌های آزاد DPPH و ABTS، قدرت کاهندگی آهن،  فعالیت شلاته کنندگی یون آهن و فعالیت آنتی‌اکسیدانی کل  در 5 غلظت متفاوت (1، 2، 3، 4 و 5 میلی‌گرم در میلی‌لیتر)، مورد ارزیابی قرارگرفت. نتایج بیان کننده آن بود که با افزایش غلظت پروتئین، خاصیت آنتی‌اکسیدانی FPH به طور معنی­داری افزایش ­یافت. بالاترین فعالیت مهارکنندگی رادیکال DPPH، قدرت کاهندگی آهن، شلاته کنندگی یون آهن و فعالیت آنتی‌اکسیدانی کل مربوط به غلظت 5 میلی­گرم بر میلی­لیتر بود. در مجموع می­توان گفت هیدرولیز آنزیمی ماهی کیلکا منجر به تولید پروتئین‌هایی با خواص آنتی‌اکسیدانی می‌شود که می­توانند بعد از تایید مطالعات کلینیکی به عنوان افزودنی مواد غذایی مورد استفاده قرار گیرند.

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