افزایش تحمل گیاهچه های مکزیکن لایم (Citrus aurantifolia Swingle ) تلقیح شده با اندوفیت‌های Aspergillus niger و Bacillus aquimaris استخراج شده از جلبک های دریایی نسبت به تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دکتری بیوتکنولوژی و ژنتیک مولکولی محصولات باغبانی، دانشگاه هرمزگان، بندرعباس، ایران

2 دانشیار گروه باغبانی، دانشکده کشاورزی و منابع طبیعی دانشگاه هرمزگان، بندرعباس، ایران

3 استادیار گروه تحقیقات گیاه‌پزشکی، مرکز تحقیقات کشاورزی و منابع طبیعی هرمزگان، سازمان تحقیقات، آموزش و ترویج کشاورزی، بندرعباس، ایران

4 استادیار مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان هرمزگان، سازمان تحقیقات، آموزش و ترویج کشاورزی، بندرعباس، ایران

10.22124/japb.2022.20908.1444

چکیده

اندوفیت­ها نقش مهمی را در محافظت از گیاهان ایفا می­ کنند. در این مطالعه، از ترکیب اندوفیتی Aspergillus niger و OD14 Bacillus aquimaris به منظور ارتقا تحمل گیاهچه ­های مکزیکن لایم نسبت به تنش شوری استفاده شد. سه ماه پس از تلقیح، تنش شوری صفر، 2000، 4000 و 6000 میکروزیمنس بر سانتی­متر (با سه تکرار) اعمال شد. پس از هشت هفته، صفات ریخت­شناسی، بیوشیمیایی و میزان جذب عناصر گیاهچه­ ها، مورد ارزیابی قرار گرفتند. نتایج نشان داد در شوری 6000 میکروزیمنس بر سانتی­متر، ترکیب اندوفیتی باعث افزایش طول ساقه (64/103 درصد)، قطر تنه (04/103 درصد) تعداد برگ (41/444 درصد) و طول و عرض ریشه (به ترتیب 02/232 و 63/41 درصد) نسبت به شاهد شد. اندوفیت باعث افزایش 29/76 درصدی کلروفیل کل نسبت به شاهد شد. همچنین، جذب عناصر پتاسیم ریشه (95/34 درصد) و کلسیم شاخساره (15/115 درصد) را افزایش و جذب عناصر سدیم شاخساره (49/29 درصد) را نسبت به شاهد کاهش داد. ترکیب اندوفیتی توانست باعث کاهش معنی­ دار نشت الکترولیت­ها (57/65 درصد) و افزایش میزان پرولین (3/69 درصد) در گیاهچه ­های تلقیح شده نسبت به شاهد شود. به طور کلی، اندوفیت­های دریایی می­توانند به عنوان گزینه مناسب برای افزایش تحمل گیاهان نسبت به تنش شوری مورد استفاده قرار گیرند.

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