ارزیابی اثر عصاره گیاه حنا (Lawsonia inermis) در مقایسه با سولفات مس (CuSo4.5H2o) بر شاخص‌های خونی، ایمنی و استرس و فعالیت آنتی‌اکسیدانی بافت کبد تاس‌ماهی سیبری (Acipenser baerii)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری شیلات، گروه شیلات، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان، ایران

2 استادیار گروه شیلات، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان، ایران

3 دانشیار گروه شیلات، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان، ایران

4 استادیار پژوهشکده آبزی‌پروری آب‌های داخلی، موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، بندرانزلی، ایران

5 استادیار انستیتو تحقیقات بین‌المللی ماهیان خاویاری، موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، رشت، ایران

10.22124/japb.2023.24825.1502

چکیده

از گیاه حنا (Lawsonia inermis) و سولفات مس (CuSO4.5H2O) به دلیل خواص آنتی‌بیوتیکی و ضدقارچی برای بهبود و ترمیم زخم‌های پوستی استفاده می‌شود. بدین منظور از 500 قطعه بچه تاس‌ماهی سیبری با میانگین وزنی 2/1±15 گرم استفاده شد. ابتدا سمیت حاد (LC50) و سپس میزان بیشترین غلظت مجاز (MAC) تعیین شد. برای انجام آزمایش، پنج تیمار با سه تکرار طراحی شد. در تیمارهای 1 تا 4 از غلظت‌هایی به اندازه مقادیر خود MAC حنا و مقادیری به اندازه 25، 50 و 75 درصد MAC استفاده شد. در تیمار 5 سولفات مس به میزان 07/0 میلی‌گرم در لیتر برای مقایسه استفاده شد. ماهیان به مدت 4 روز (96 ساعت) تحت تاثیر ترکیبات با غلظت‌های تعیین‌شده قرار گرفتند. نتایج نشان داد که عصاره گیاه حنا در شاخص‌هایی همچون تعداد گلبول سفید، کاهش استرس، عملکرد آنزیم‌های کبدی عملکرد مناسب‌تری نسبت به سولفات مس از خود نشان داد. نهایتا مطالعه حاضر نشان داد عصاره گیاه حنا بهتر عمل کرد و می‌تواند به عنوان جایگزین مناسبی برای سولفات مس برای ضدعفونی در مزارع پرورش تاس‌ماهی سیبری مورد استفاده قرار گیرد. همچنین نتایج نشان دادکه عصاره گیاه حنا دارای تاثیرات منفی بر شاخص­های خونی و بیوشیمیایی تاس‌ماهی سیبری بود.

کلیدواژه‌ها

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