Effect of hCG, Ovaprim™ and OvarimTM on the efficiency of artificial reproduction of European perch (Perca fluviatilis) broodstock

Document Type : Research Paper

Authors

1 M.Sc. in Fisheries, Shahid Ansari Teleost Fish Restocking and Genetic Conservation Center, Rasht, Iran

2 M.Sc. in Fisheries, Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran

3 Ph.D. in Fisheries, Department of Aquaculture, Inland Water Aquaculture Research Centre, Iranian Fisheries Science Research Institute, Agriculture Research Education and Extension Organization (AREEO), Bandar-e Anzali, Iran

4 Professor in Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran

5 Professor in Department of Marine Sciences, The Caspian Sea Basin Research Center, University of Guilan, Rasht, Iran

10.22124/japb.2023.24539.1497

Abstract

The present study was performed to determine the effect of hCG, Ovaprim, and Ovarim on the efficiency of artificial reproduction in European perch (Perca fluviatilis), as well as the examination of the spermatological parameters of this fish. In the first treatment, hCG was used at 500 IU per kg body weight (BW) of broodstock. In second and third treatments, Ovaprim and Ovarim were used at 0.5mL per kg BW, respectively. The control group was injected with physiological saline at 0.5mL per kg BW. Eighteen female broodstocks with an average weight of approximately 78g were used in each treatment. Male broodstock was induced by injection of hCG at a dose of 250 IU per kg BW. According to the results, the fastest latency time (415.6 degree-hour) was observed in the hCG treatment and showed a significant difference with other treatments (P<0.05). The highest ovulation rate (100%) was observed in hCG and Ovaprim treatments and showed significant differences with Ovarim and control groups (P<0.05). The experimental treatments did not show any significant differences in other parameters (P>0.05). Based on the results, the mean volume of semen, spermatozoa activity, motility time and spermatocrit value of European perch were 2.9±1.7mL, 91.1±13.4%, 84.4±18.6s and 68.3±9.4%, respectively. Results of this study showed that hCG is more suitable than Ovaprim and Ovarim for the induction of spawning and artificial reproduction of European perch. Due to the more favorable effects of hCG, it is recommended to use this hormone in spawning induction and artificial reproduction of European perch broodstock.

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Main Subjects

References

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Aquatic Physiology and Biotechnology
Volume 12, Issue 1
July 2024
Pages 67-82

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