Investigating the bioremoval ability of crude oil by bacteria isolated from oil-contaminated soil around Shiraz refinery

Document Type : Research Paper

Authors

1 M.Sc. in Environmental and Industrial Biotechnology, Department of Marine Biology, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran

2 Professor in Department of Biology, Faculty of Sciences, University of Qom, Qom, Iran

3 Associate Professor in Department of Marine Biology, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran

10.22124/japb.2023.24454.1496

Abstract

Today, oil pollution has evolved into a global environmental concern. One of the methods for mitigating this pollution is the use of biological approaches, especially microorganisms, known for their cost-effectiveness and environmental friendliness, rendering them particularly popular among various methods. This study aimed to explore the potential growth of oil-degrading bacteria in oil-contaminated soils, as well as their ability to remove crude oil. Based on bacterial growth rates in the presence of crude oil and hydrocarbon degradation capabilities, two strains, Sludge C and SHA, were selected from among 13 isolates obtained from oil-contaminated soil near the Shiraz refinery. Subsequently, they were assessed for oil degradation using spectrophotometry and gas chromatography methods. Results indicated that Sludge C and SHA possess oil consumption abilities of 64.4% and 84.2%, respectively, within 7 days. Gas chromatography analysis highlighted SHA's remarkable capability in removing short, medium, and long-chain hydrocarbons. Molecular identification confirmed the affiliation of this strain with the genus and species Acinetobacter junii. With its high oil consumption potential, this strain holds promise for efficient utilization in environmental remediation and industry.

Keywords

References

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Aquatic Physiology and Biotechnology
Volume 12, Issue 1
July 2024
Pages 109-130

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