Evaluation of the effect of henna plant (Lawsonia inermis) extract compared to copper sulfate (CuSo4.5H2o) on blood, immunity and stress indices and antioxidant activity in liver tissues of Siberian sturgeon (Acipenser baerii)

Document Type : Research Paper

Authors

1 Ph.D. Student in Fisheries, Department of Fisheries, Lahijan Branch, Islamic Azad University, Lahijan, Iran

2 Assistant Professor in Department of Fisheries, Lahijan Branch, Islamic Azad University, Lahijan, Iran

3 Associate Professor in Department of Fisheries, Lahijan Branch, Islamic Azad University, Lahijan, Iran

4 Assistant Professor in Inland Water Aquaculture Research Institute, National Fisheries Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Anzali, Iran

5 Assistant Professor in International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

10.22124/japb.2023.24825.1502

Abstract

Henna plants (Lawsonia inermis) and copper sulfate (CuSO4.5H2O) are used for healing and repairing skin wounds due to their antibiotic and antifungal properties. For this purpose, 500 pieces of Siberian sturgeons with an average weight of 15±1.2g were used. First, the acute toxicity (LC50) and then the maximum permissible concentration (MAC) were determined. To experiment, five treatments with three replications were designed. In treatments 1 to 4, doses equal to henna MAC value and amounts equal to 25%, 50%, and 75% of MAC were used. In treatment 5, copper sulfate at the concentration of 0.07 mg/L was used to compare. Fish were affected by compounds with determined concentrations for 4 days (96 hours). The results showed that henna plant extract performed better than copper sulfate in indicators such as white blood cell count, stress reduction, and liver enzyme activity. Finally, the present study showed that henna plant extract performed better and can be used as a suitable substitute for copper sulfate for disinfection in Siberian sturgeon farms. Also, the results showed that henna plant extract has negative effects on the blood and biochemical indices of Siberian sturgeon.

Keywords

Main Subjects

References

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Aquatic Physiology and Biotechnology
Volume 12, Issue 1
July 2024
Pages 131-159

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