Assessment of bacterial flora probiotics activity isolated from the water and sediments of silver carp (Hypophthalmichthys molitrix) broodstocks ponds

Document Type : Research Paper

Authors

1 Associate Professor in Aquatics and Fisheries Department, Guilan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

2 M.Sc. in Environmental Research Department, Guilan Environmental General Office of Environment, Iranian Environment Organization, Rasht, Iran

3 Assistant Professor in Aquatics and Fisheries Department, Guilan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

10.22124/japb.2025.30430.1569

Abstract

This study was conducted to screen water and sediments bacterial flora of silver carp broodstock including Bacillus subtilis, Escherichia coli, Corynebacterium ammoniagenes, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus luteus, Aeromonas hydrophila, and Unidentified as probiotics. Antibacterial activities were conducted by using disc diffusion, well diffusion and cross streak methods under the influence of different pH, salt, temperature and time duration. The 96h effectiveness of a suspension of three probiotic candidate species and the pathogenic bacterium A. hydrophila was investigated on the mortality rate of silver carp larvae with different concentrations of candidate bacteria (105 and 107 cells/mL) and a control without bacteria in a flask (1L). Also, effectiveness of selected bacteria was investigated on the water quality and survival rate of silver carp fry in the treatment and control groups during 28 days. The three probiotics candidates Bacillus, Corynebacterium and Micrococcus were shown to have antibacterial activity against pathogenic bacteria. The optimal growth and antibacterial bio-components production of selected B. subtilis with inhibitory and lethal effectiveness characteristics against A. hydrophila were found at pH 8 and temperature 30 and 37°C. There were significant differences between treatments and control groups in larva mortality rates during 96h exposure to different concentrations of candidate bacteria (P<0.05). There were significant differences in water quality and growth indices between treatments and control groups during 28 days exposure to selected B. subtilis in aquaria (P<0.05). From this, we can conclude that the candidate B. subtilis can be used to improve water quality and growth of silver carp culture.

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References

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Aquatic Physiology and Biotechnology
Volume 13, Issue 4
April 2026
Pages 49-72

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