Establishment and characterization of epithelial-like cell line from Caspian trout (Salmo trutta caspius) fin tissues

Noroozi, Kaveh; Kallbassi, Mohammad Reza; Farzaneh, Parvaneh; Shahzadeh Fazeli, Seyed Abolhasan; Farghadan, Maryam; Nasimian, Ahmad; Izadpanah, Mehrnaz; Ashouri Movassagh, Sepideh; Mohammadi, Shiva; Moradmand, Zahra; Farhangnia, Mansoureh

Establishment and characterization of epithelial-like cell line from Caspian trout (Salmo trutta caspius) fin tissues

Document Type : Research Paper

Authors

Abstract

Cell lines provide an important biological tool for carrying out investigations into physiology studies and their applications are more growing every day. Here we tried to establish a cell line from Caspian salmon (Salmo trutta caspius). We used Caspian salmon fry and two primary culture methods, Explant and Enzymatic disaggregation were undertaken. Cell migration from pectoral, dorsal, caudal and anal fins was studied. Leibovitz’s L-15 with 20% FBS and 1% Pen-Strep was used as culture medium. Karyotyping was conducted to characterization of established cell line. Consequently cells were freezed with DMSO and stored at -196˚C. Explant Efficiency was showed that the best fin for explant is pectoral fin with the average of 71.31%. In comparison between two primary culture methods, enzymatic disaggregation with trypsin was more efficient. Chromosome counts were suggested 2n=80 and viability was 84.4% after one month storage in liquid nitrogen. Consequently, these cells have been subcultured, Freezed and defreezed over 22 times since their initiation and showed reasonable growth rate, therefor they can be used as cell lines in other studies. These cell lines can be reached at Iranian Biological Resource Center (IBRC) as the first aquactic animal cell strain with short names; CS2-1,CS2-2, CSe and IBRC C10190-3 catalogue numbers.

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