Impact assessment of potassium permanganate and copper sulfate on hematological and stress indices in Acipenser ruthenus

Document Type : Research Paper

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Abstract

External disinfectants are used to control infections caused by organisms outside the body and to eradicate or reduce pathogens in fish. The present study investigates the effect of different copper sulfate (CuSO4.5H2O) and potassium permanganate (KMnO4) baths on stress and blood indices of Acipenser ruthenus. The experiment was carried out in a completely randomized design with 6 treatments and a control group (triplicate). Treatments were copper sulfate concentrations of 2ppm (long-term bath, 12h), 5ppm (short-term bath, 5-10min), 10ppm (bath of immersion, 30-45s) and potassium permanganate concentrations of 1ppm (long-term bath, 12h), 2ppm (short-term bath, 5-10min), 3ppm (bath of immersion, 30-45s) and a control group (without sterilization). At the end of experiment, 4 to 5 fish had randomly selected for blood sampling. White blood cell and Neutrophil results showed a statistically significant difference between treatments and control group (P<0.05). Lymphocyte, monocytes and eosinophils percentage, mean corpuscular volume, mean corpuscular hemoglobin concentration and mean corpuscular hemoglobin didn’t show a statistically significant difference between treatments and control group (P>0.05). Cortisol and glucose levels showed a statistically significant difference between treatments and control group (P<0.05). According to the results of the disinfection method, disinfection short-term (10 min) is recommended for both materials.

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