The Protective effect of vitamin C on DNA breakage of gills and liver cells in Rainbow trout (Oncorhynchus mykiss) exposed to different concentrations of zinc oxide nanoparticles (ZnO)

Document Type : Research Paper

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Abstract

This study was to evaluate the effect of emerging pollutant nanoparticles of zinc oxide (ZnO) and safety effects of vitamin C on the DNA breakage in the liver and gill cells of rainbow trout (Oncorhynchus mykiss). Fish were exposed to nanoparticles on three levels (0, 40 and 80 mg/L) and three levels of vitamin C (0, 400 and 800 mg/Kg) in 9 treatments with 3 replications. Fish with average weight 170±10g were treated in 10 days. Liver and gill tissues were sampled in the fifth and tenth days. After the isolation, target samples were stored in microtubes contain alcohol 96%. DNA extraction was run with gel electrophoresis. At the end of the test, it was photographed with Gel-doc. Breakage of DNA was determined using the weighted average. According to the results, in liver and gill, effect of zinc oxide nanoparticle, vitamin C and interaction with time-concentration were quite significant on the level of DNA damage. Most damage on liver and gill tissues in 80 mg/L of zinc oxide nanoparticles were on the 5th day. Also in both tissues, DNA breakage rate was on the 10th day.  However, vitamin C was reduced DNA breakage.

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Aquatic Physiology and Biotechnology
Volume 4, Issue 4
March 2017
Pages 111-132

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