CYP1A gene sequencing and Study of gene expression pattern in different tissues of mudskipper (Periophthalmus waltoni) captured from natural habitat

Document Type : Research Paper

Authors

1 Assistant Professor in Biotechnology Department, Persian Gulf Research Center, University of Persian Gulf, Boushehr, Iran

2 Associate Professor in Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran

3 Professor in Department of Parasitology, Faculty of Medicine, Shaheed Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Cytochrome CYP1A is one of the main involved enzymes in the metabolism of the xenobiotic. Identifying the cytochrome P450 (CYP1A), responsible for the major metabolic pathways, can lead to understanding the metabolism pathway of organic materials and PAHs adaptation of sea fish. Results from cloning and identifying CYP1A gene in Periophthalmus waltoni showed CYP1A contained 1035 nucleotide from open reading frame sequenced. CYP1A basal gene expression was varied from 23, 22, 19, 17, 11, 11% for gill, kidney, heart, intestine, brain respectively. According to the results, liver organ showed the highest response of CYP1A gene expression like other fish species. But, the basic gene expression of CYP1A in other organs in comparison with liver organ was higher. Although basal expression pattern of genes depending on the species, high basal expression of gene CYP1A due to P. waltoni was exposure to PAH in natural conditions. Different CYP1A gene expression pattern in different organs showed ecophysiological condition of mudskipper. So, P. waltoni has the potential to be used as an exact biomarker for evaluation of organic pollutant of ecosystems especially PAHs.

Keywords

References

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Aquatic Physiology and Biotechnology
Volume 5, Issue 1
June 2017
Pages 69-86

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