Document Type : Research Paper
Authors
1
Ph.D. Student in Fisheries, Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran.
2
Professor in Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran
3
Professor in Department of Marine Sciences, Caspian Sea Basin Research Center, University of Guilan, Rasht, Iran
4
Associate Professor in Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran
5
Associate Professor in Department of Marine Sciences, The Caspian Sea Basin Research Center, University of Guilan, Rasht, Iran
6
Assistant Professor in Inland Waters Aquaculture Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Anzali, Iran
7
Associate Professor in Department of Fisheries, Lahijan Branch, Islamic Azad University, Lahijan, Iran.
8
Assistant Professor in Department of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Abstract
The present study purposed to determine the dose- and time-dependent changes in digestive enzyme activity (lipase, amylase and protease) and intestine histopathological alterations in Persian sturgeon, Acipenser persicus after exposure to sublethal concentrations (0, 0.05, 0.1 and 0.2 LC50) of malathion for 7 days. Based on the results, the activity of lipase, amylase and protease levels substantially decreased in the fish exposed to malathion (P<0.05). These activities were significantly dose- and time-dependent (P<0.05) and the lowest was in 0.2mgL-1 at the end of the experiment. Histological studies of the processed tissues showed marked histological alterations than the control group. The pathologic lesions of the intestine included blood congestion, loss of epithelial cells, fusion of microvilli, loss of microvilli, sloughing of epithelial cells, flattening of intestinal microvilli, vacuolization of cell cytoplasm, hypertrophy of epithelial cells, shrinkage of microvilli and also cell necrosis in muscular were observed exposure to sublethal concentrations of malathion. In most cases, the severity of changes follows a dose- and time-dependent manner. Results showed that low concentration of malathion (0.05 LC50) can pose undesirable alternation in Persian sturgeon fingerling, an economic species of the Caspian Sea.
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