Document Type : Research Paper
Authors
1
M.Sc. Student in Fisheries, Department of Fisheries, Faculty of Agriculture Science and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran
2
Assistant Professor in Department of Fisheries, Faculty of Agriculture Science and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran
3
Assistant Professor in Food Hygiene and Public Health Department, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
4
Associate Professor in Department of Fisheries, Faculty of Agriculture Science and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran
Abstract
This study aimed to evaluate C-type lysozyme gene expression in the liver, spleen, pancreas and head kidney of breeding Acipenser persicus based on gender with real-time PCR method. For this purpose, sampling of liver, spleen, pancreas and head kidney was done after artificial breeding from 16 fish including 8 males and 8 females. The tissues were transported to the laboratory in liquid nitrogen. After RNA extraction and cDNA synthesis, quantitative real-time PCR was done to examine the relevant gene expression in different tissues. The finding as the first record in this species showed that the C-type lysozyme gene was expressed in all investigated tissues and the expression rate between different tissues had a significant difference (P<0.01). The highest and lowest expression level of mRNA was observed in spleen and pancreas tissues, respectively. The expression of this gene in different gender of these species showed a significant difference (P<0.01). So, the expression rate of the C-type lysozyme gene in male tissues was significantly higher than females. Generally, according to the results, it can be stated that the expression rate of the C-type lysozyme gene in different tissues and genders of breeding A. persicus can be influenced by different factors whose potential effects may require further laboratory studies on this filed.
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