Investigation of gene expression of C-type lysozyme from spleen, pancreas, anterior kidney and liver tissues in adult Persian sturgeon (Acipenser persicus Borodin, 1897) by using the quantitative real-time PCR Method

Document Type : Research Paper

Authors

1 M.Sc. Student in Fisheries, Department of Fisheries, Faculty of Agriculture Science and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran

2 Assistant Professor in Department of Fisheries, Faculty of Agriculture Science and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran

3 Assistant Professor in Food Hygiene and Public Health Department, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

4 Associate Professor in Department of Fisheries, Faculty of Agriculture Science and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran

Abstract

This study aimed to evaluate C-type lysozyme gene expression in the liver, spleen, pancreas and head kidney of breeding Acipenser persicus based on gender with real-time PCR method. For this purpose, sampling of liver, spleen, pancreas and head kidney was done after artificial breeding from 16 fish including 8 males and 8 females. The tissues were transported to the laboratory in liquid nitrogen. After RNA extraction and cDNA synthesis, quantitative real-time PCR was done to examine the relevant gene expression in different tissues. The finding as the first record in this species showed that the C-type lysozyme gene was expressed in all investigated tissues and the expression rate between different tissues had a significant difference (P<0.01). The highest and lowest expression level of mRNA was observed in spleen and pancreas tissues, respectively. The expression of this gene in different gender of these species showed a significant difference (P<0.01). So, the expression rate of the C-type lysozyme gene in male tissues was significantly higher than females. Generally, according to the results, it can be stated that the expression rate of the C-type lysozyme gene in different tissues and genders of breeding A. persicus can be influenced by different factors whose potential effects may require further laboratory studies on this filed.

Keywords

Aquatic Physiology and Biotechnology
Volume 7, Issue 3
October 2019
Pages 161-186

Files

Share

How to cite

Statistics