Screening of lipase-secreting marine halophilic Actinobacteria from oil-contaminated sediments of hypersaline Lake Urmia

Document Type : Research Paper

Authors

1 Ph.D. Student in Microbiology, Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran

2 Assistant Professor in Department of Biology, Faculty of Science, Urmia University, Urmia, Iran

3 Assistant Professor in Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran

Abstract

In this study, the lipases-producing Actinobacterial species were isolated from oil-contaminated sites of the hypersaline Lake Urmia. All Actinobacteria isolates were examined for lipase activity by Tween-20 and olive oil as lipid substrates. After DNA extraction, the isolated strains were identified by 16S rRNA gene analysis. Six isolates of halophilic Actinobacteria named LUG03, LUS05, LUG09, LUN15, LUG21 and LUB22 were isolated from the salt sediments of Lake Urmia (Bandar Sharafkhaneh). Among them four isolates showed high potential for lipase production with the highest lipolytic activity for LUS05 strain. Sequence analysis of 16S rRNA identified that the isolates belonged to Streptomyces sp., Nocardiopsis sp., Glutamicibacter sp. and Brachybacterium sp.

Keywords

References

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Aquatic Physiology and Biotechnology
Volume 9, Issue 3
December 2021
Pages 47-66

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