Ionocyte changes in the skin of Sander lucioperca larvae exposed to different salinities

Document Type : Research Paper

Authors

1 Assistant Professor in Inland Water Aquaculture Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Anzali, Iran

2 Professor in Biology Department, Biology Faculty, Kharazmi University, Tehran, Iran.

3 Professor in Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

4 Associate Professor in Inland Waters Aquaculture Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Anzali, Iran

Abstract

The aim of the present study was to evaluate the osmoregulation ability of zander (Sander lucioperca) at two larval stages including before (group “a”) and after (group “b”) yolk sac absorption. Both groups were exposed to freshwater (less than 0.5‰ salinity), estuarine conditions (7‰) and Caspian Sea water (12‰) for 96h. Larva survival rate and chloride cell number, size and the its surface percentage were examined by classical histology using H & E staining and immunolocalization of Na+,K+-ATPase. Survival rate was 97% for both groups in freshwater and was 86% and 97%, respectively for group “a” and “b” at 7‰ salinity. At 12‰ salinity, all larvae in group “a” died while 20% of group “b” survived. The size of skin chloride cells did not change in the face of different salinity. At the end of the experiment, the number and the surface percentage of chloride cells significantly increased in the group “a” at 7‰ salinity and in group “b” at 12‰ salinity (P<0.05). The results showed that in the Caspian Sea zander larvae, the function of skin chloride cells in osmoregulation improved with development of body and it could act in such a way that the survival rate in the post-yolk sac uptake stage was more than the pre-yolk sac uptake stage exposed to salinity.

Keywords

References

  احمدنژاد م.، عریان ش.، بهمنی م. و صیاد بورانی م. 1393. روند ظهور یونوسیت‌های آبشش و پوست در مراحل اولیه رشد و نمو لارو لارو سوف سفید دریای خزر (Sander lucioperca). فصلنامه علمی پژوهشی فیزیولوژی و تکوین جانوری، 7(2): 48-39.
عبدلی ا. 1378. ماهیان آب‌های داخلی ایران. انتشارات موزه طبیعت و حیات وحش ایران. 377ص.
Ayson F.G., Kaneko T., Hasegawa S. and Hirano T. 1994. Development of mitochondrian rich cells in the yolk sac membrane of embryos and larvae of Tilapia (Oreochromis mossambicus) in fresh water and sea water. Journal of Experimental Zoology, 270: 129–135.
Bancroft J.D. and Stevens A. 1977. Theory and Practice of Histological Techniques. Churchill Livingstone, UK. 436P.
Bodinier C., Sucre E., Lecurieux-Belfond L., Blondeau-Bidet E. and Charmantier G. 2010. Ontogeny of osmoregulation and salinity tolerance in the gilthead sea bream Sparus aurata. Comparative Biochemistry and Physiology (A), 157: 220–228.
Craig J.F. 2000. Percid fishes: Systematic, Ecology and Exploitation. Blackwell Science, USA. 352P.
Evans T.G. 2010. Co-ordination of osmotic stress responses through osmosensing and signal transduction events in fishes. Journal of Fish Biology, 76: 1903–1925.
Ghanizadeh Kazerouni E. and Khodabandeh S. 2011. Ionocyte immunolocalization and the effects of ultraviolet radiation on their abundance and distribution in the alenins of Caspian Sea salmon, Salmo trutta caspius. Cell Journal (Yakhteh), 13(1): 45–54.
Hamza N., Mhetli M., Khemis I.B., Cahu C. and Kestemont P. 2008. Effect of dietary phospholipid levels on performance, enzyme activities and fatty acid composition of pikeperch (Sander lucioperca) larvae. Aquaculture, 275: 274–282. 
Hiroi J., Kaneko T., Seikai T. and Tanaka M. 1998. Developmental sequence of chloride cells in the body skin and gills of Japanese flounder Paralichthyys olivaceus larvae. Zoological Science, 15: 455–460.
Hiroi J., McCormick S.D., Ohtani-Kaneko R. and Kaneko T. 2005. Functional classification of mitochondrion rich cells in euryhaline Mozambique tilapia Oreochromis mossambicus embryos by means of triple immunofluorescence staining for Na+,K+-ATPase, cotransporters and CFTR anion channel.  Journal of Experimental Biology, 208: 2023–2036.
Hirose S., Kaneko T., Naito N. and Takei Y. 2003. Molecular biology of major components of chloride cells. Comparative Biochemistry and Physiology, 136: 593–620.
Hwang P.P. 1990. Salinity effects on development of chloride cells in the larvae of ayu (Plecoglossus altivelis). Marine Biology, 107: 1–7.
Hwang P.P., Sun C.M. and Woo S.M. 1989. Changes of plasma osmolarity, chloride concentration and gill Na+-K+-ATPase activity in tilapia Oreochromis mossambicus during seawater acclimation. Marin Biology, 100: 295–233.
Kaneko T. and Hiroi J. 2008. Osmo and ionoregulation. P: 163–183. In: Finn R.N. and Kapoor B.G. (Eds.). Fish Larval Physiology. Science Publishers, USA.
Kaneko T., Shiraishi K., Katoh F., Hasegawa S. and Hiroi J. 2002. Chloride cells during early life stages of fish and their functional differentiation. Fisheries Science, 68: 1–9.
Kaneko T., Watanabe S. and Kyung Min L. 2008. Functional morphology of mitochondrion- rich cells in euryhaline and stenohaline teleosts. Aqua Bioscience Monographs, 1(1): 1–62.
Khodabandeh S., Charmantier G. and Chramantier-Daures M. 2006. Immunolocalization of Na+,K+-ATPase in osmoregulatory organs during the embryonic and post-embryonic development of the lobster, Homarus gammarus. Journal of Crustacean Biology, 26(4): 515–523.
Khodabandeh S., Mosafer S. and Khoshnood Z. 2009a. Effect of cortisol and salinity acclimation on Na+,K+,2Cl- cotransporter gene expression and Na+,K+-ATPase activity in gill of  Persian sturgeon Acipenser persicus fry. Scietia Marina, 73: 111–116.
Khodabandeh S., Shahriari M. and Abtahi B. 2009b. Changes in chloride cells abundance Na+,K+-ATPase immunolocalization and activity in gills of golden grey mullet, Liza aurata fry during adaptation to different salinities. Yakhteh Medical Journal, 11: 49–54.
Lasker R. and Threadgold L.T. 1968. Chloride cells in the skin of the larval sardine. Experimental Cell Research, 52: 582–590.
Lignot J.H., Nugroho susanto G., Charmantier-daures M. and Charamantier G. 2001. Immunolocalization of Na+,K+-ATPase in the branchial cavity during the early development of cray fish Astacus leptodactylus (Crustacea, Decapoda). Cell and Tissue Research, 319: 331–339.
Nebel C., Romestand B., Negre-Sadargues G., Grousset E., Aujoulat F., Bacal J., Bonhomme F. and Charmantier G. 2005. Differential freshwater adaptation in juvenile sea bass Dicentrarchus labrax: Involvement of gills and urinary system. Journal of Experimental Biology, 208: 3859–3871.
O'connell C.P. 1981. Development of organ systems in the northern anchovy Engraulis mordax and other teleosts. American Zoology, 21: 429–446.
Oguz A.R. 2018. Development of osmoregulatory tissues in the Lake Van fish (Alburnus tarichi) during larval development. Fish Physiology and Biochemistry, 44: 227–233.
Rambourgh P.J. 1999. The gill of fish larvae. Is it primarily a respiratory or an ionoregulatory. Journal of Fish Biology, 55(A): 186–204.
Shelbourne J.E. 1957. Site of chloride regulation in marine fish larvae. Nature, 180: 920–922.
Shikano T. and Fujio Y. 1999. Changes in salinity tolerance and branchial chloride cells of newborn guppy during freshwater and seawater adaptation. Journal of Experimental Zoology, 284: 137–146.
Van Der Heijden A.J.H., Van Der Meij J.C.A., Flik G. and Wendelaar Bonga S.E. 1999. Ultra-structure and distribution dynamics of chloride cells in tilapia larvae in fresh water and sea water. Cell Tissue Research, 297: 119–130.
Varsamos S., Connes R., Diaz J.P., Barnabe G. and Charmantier G. 2001. Ontogeny of osmoregulation in the European sea bass Dicentrarchus labrax L. Marine Biology, 138: 909– 915.
Varsamos S., Nebel C. and Charmantier G. 2005. Ontogeny of osmoregulation in postembryonic fish: A review. Comparative Biochemistry and Physiology (A), 141: 401–429.
Wales B. and Tytler P. 1996. Changes in chloride cell distribution during early larval stages of Clupea harengus. Journal of Fish Biology, 49: 801–814.
Aquatic Physiology and Biotechnology
Volume 9, Issue 3
December 2021
Pages 149-169

Files

Share

How to cite

Statistics