The effect of salinity changes on the liver enzyme activity and malondialdehyde biomarker in gray mullet (Mugil cephalus)

Document Type : Research Paper

Authors

1 M.Sc. in Marine Biology, Department of Marine Biology, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran

2 Associate Professor, Department of Marine Biology, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran

3 Assistant Professor, Department of Marine Biology, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran

10.22124/japb.2024.26521.1525

Abstract

The present study was conducted with the aim of investigating the effect of salinity changes on the activity of liver enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and the biomarker malondialdehyde (MDA) in gray mullet. Fish samples, after acclimatization, were studied in treatments of 28, 34, 50g/L and control (36.5g/L) in three periods of 7, 14 and 21 days. Results showed that malondialdehyde biomarker was the highest on the 7th and 14th days in 50g/L salinity treatment, but the highest value was recorded on the 21st day in 34g/L salinity. The statistical test showed a significant difference between days and treatments (P<0.05). Alanine aminotransferase had the highest value on the 7th day at 50g/L salinity, 14th day at the control and 21st day at 28g/L salinity, and the difference between the data was significant. Aspartate aminotransferase enzyme had the highest amount on the 7th and 21st days of the control treatment and on the seventh day of 34g/L salinity. The control treatment had always a significant difference with other treatments. Alkaline phosphatase enzyme had the highest value on the 7th day at 34g/L salinity, 14th day at 28g/L salinity and 21st day at 50g/L salinity, and except for 50g/L salinity, the concentration of the enzyme decreased significantly from day 7 to 21 in other treatments. No significant correlation was observed between any of the measured parameters and salinity. The general result showed that in most of the treatments, due to the increase in salinity, the concentration of enzymes had a decreasing trend, which indicated the inhibition of enzyme activity in high salinity. In addition, reducing salinity showed an increasing effect to deal with stress and create adaptation.

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Main Subjects

References

 
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Aquatic Physiology and Biotechnology
Volume 12, Issue 3
February 2025
Pages 97-116

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