Microbial treatment of wastewater from great sturgeon culture (Huso huso ) tanks under influence of different concentrations of Bacillus , Corynebacterium , Nitrosomonas and Nitrobacter bacterial composition in the water recirculation system

Document Type : Research Paper

Authors

1 Ph.D. Student in Fisheries, Department of Fisheries, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran

2 Professor in Department of Fisheries, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran

3 Associate Professor in Fisheries and Aquatics Department, Mirzakochek Khan Fisheries Sciences and Industries Training Unit, Guilan Center, Agricultural Research, Education and Extension Organization Rasht, Iran.

4 Associate Professor in Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran

10.22124/japb.2024.26682.1530

Abstract

This study was conducted with designed biofilters containing a combination of Bacillus, Corynebacterium, Nitrosomonas and Nitrobacter bacteria at different concentrations and a control (without bacteria) for biological treatment of wastewater from aquariums with a water intake volume of 0.4m3 and a density of 10 great sturgeon (Huso huso). The inlet water flow rate of each aquariums was 0.004L/s and the number of water circulation times of each aquariums was determined once in 24 hours. 4 days after adding fish with an average weight of 21.35±2.67g to the aquariums, a mixture of 4 bacteria in the form of concentrated suspensions at ratios of 105, 107 and 109 cells/mL was inoculated into the biofilters placed at the effluent outlet of these aquariums. The levels of ammonia, nitrite, nitrate, weight and growth indices of the fry were evaluated weekly. After 4 weeks of treatment with different concentrations of the selected bacterial composition, the difference in water quality between the experimental and control treatments was significant (P<0.05). During the experimental period, the levels of pH, oxygen, ammonia, nitrite and nitrate increased in the control treatment and decreased in the three experimental treatments. The survival rate, weight gain and specific growth rate increased in the experimental treatments compared to the control. On the contrary, the feed conversion ratio in the control treatment was higher than the experimental treatments (P<0.05). However, comparing the experimental treatments with each other, there was no statistically significant difference between them (P>0.05). Therefore, based on these results, the selected bacterial composition cultivated in biofilters with a concentration of 107 cells/mL can be used to improve the quality of sturgeon farm effluent.

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Main Subjects

References

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Aquatic Physiology and Biotechnology
Volume 12, Issue 4
March 2024
Pages 39-56

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