Synergistic effects of titanium dioxide nanoparticles, L-carnitine, HUFA and lecithin on the proliferation of industrial yeast Saccharomyces cerevisiae

Document Type : Research Paper

Authors

1 Associate Professor in Department of Fisheries, Faculty of Natural Resources, Urmia University, Urmia, Iran

2 M.Sc. in Biotechnology, Department of Biology, Faculty of Sciences, Urmia Branch, Islamic Azad University, Urmia, Iran

3 Ph.D. Student in Fisheries, Department of Production and Utilization of Aquatics, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

4 M.Sc. in Marine Biotechnology, Department of Fisheries, Faculty of Natural Resources, Urmia University, Urmia, Iran

10.22124/japb.2025.28879.1556

Abstract

The possibility of stimulating the proliferation of Saccharomyces cerevisiae yeast cells by adding titanium dioxide (TiO2) nanoparticles, HUFA, lecithin and L-carnitine was investigated in this study. After initial activation in the basal culture medium, the yeast was first individually exposed to different concentrations of these substances, and the best concentrations were selected. Then, the best individual concentrations were used to investigate the combined effect of these substances on yeast growth. In both stages, the rate of yeast cell proliferation was measured by measuring the number of cells obtained and the amount of biomass produced, as well as the percentage of yeast fatty acids in the best treatments using gas chromatography. The results showed that TiO2 at a concentration of 80mg/L significantly increased yeast proliferation. However, the best yeast growth in the presence of L-carnitine was related to concentrations of 0.01 and 0.05mg/mL. While HUFA alone did not have a good effect on yeast proliferation, the combination of HUFA with lecithin was able to have good results in yeast proliferation. Also, the combination of nanoparticles and L-carnitine was able to stimulate the proliferation of yeast cells (P<0.05). The study of the percentage of fatty acids of successful treatments showed that in all treatments, the amount of HUFA and PUFA was greatly improved compared to the control (P<0.05). However, in the study of biomass, the best individual treatment was obtained for L-carnitine with a weight of 0.67g and the combination treatment of HUFA with lecithin with 0.75g. The results of this study can be used for the cultivation of yeast types with low proliferation ability.

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References

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Aquatic Physiology and Biotechnology
Volume 13, Issue 3
January 2026
Pages 81-104

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