Investigating the antioxidant properties of jellyfish venom Chrysaora hysoscella

Mohammadzadeasl, Asghar; Zolgharnein, Hossein; Matroodi, Soheila

doi:10.22124/japb.2024.26396.1523

Investigating the antioxidant properties of jellyfish venom Chrysaora hysoscella

Document Type : Research Paper

Authors

¹ Department of Marine Biology, Faculty of Marine and Ocean Sciences, Khorramshahr University of Marine Sciences and Arts, Khorramshahr, Iran

² Department of Marine Biology, Faculty of Marine and Ocean Sciences, Khorramshahr University of Marine Sciences and Technologies, Khorramshahr, Iran

10.22124/japb.2024.26396.1523

Abstract

The venom of jellyfish species contains compounds including some potential therapeutic properties. In this regard, the venom of Chrysaora hysoscella jellyfish has various properties that have been less discussed. In this research, the extraction and purification of C. hysoscella jellyfish venom and its antioxidant properties are investigated. Then its fractions were purified using high performance liquid chromatography (HPLC). Then the purified proteins were evaluated using SDS-PAGE. Next, the antioxidant effect of the fractions was studied using three methods: DPPH, ABTS and FRAP. The results showed that the raw venom of C. hysoscella contains 10 fractions with a molecular weight of 15 to 170 kilodaltons. Among these, one of the fractions had strong antioxidant properties. Antioxidant property with DPPH, ABTS and FRAP tests showed that fraction 2 in concentrations of 31, 21 and 52 μg/ml is capable of inhibiting 50% of free radicals, which confirms the antioxidant property. It is a strong oxidant. In the present research, it was found that the venom of C. hysoscella jellyfish has a fraction with strong antioxidant properties.

Keywords

Main Subjects