The effect of extracted peptides from sea anemone Stichodactyla haddoni mucus on gill tissue, plasma osmolarity and ions of rainbow trout Oncorhynchus mykiss fry

Document Type : Research Paper


Department of Aquaculture, Marine Sciences Faculty, Tarbiat Modares University, Noor, Mazandaran, Iran.


In this study, the extraction of peptides from sea anemone Stichodactyla haddoni mucus andtheir effects on the gills of rainbow trout Oncorhynchus mykisswere examined. For this purpose, Specimens of S. haddoni were collected from the intertidal coast from Hormuz Island. Frozen samples were transported to the laboratory.Then the mucus was extracted using of the PBS solvent.Peptides down of 10kDa were separated by amicon® Ultra-15 10K millipore filter device. These peptides were injected into the caudal vein of the fish and after inactived, histopathological changes were examined using of the classical histological and immunohistochemical method and also plasma osmolarity and ions were examined in this fish. The various tissue damages of the gill were observed including hypertrophy, lamella deformation, subepithelial edema, lamella congestion in the interlamellar region and necrosis. The injection of these peptides also reduced blood plasma ions and osmolarity. The results showed that the extracted peptides from sea anemone mucus can cause numerous anomalies in the gill tissue, change in blood plasma ions and osmolarity of rainbow trout and finally resulted in failure of physiological osmoregulation function of the gills and finally led to the fish death.