Investigation of muscle histopathological indices in goldfish (Carassius auratus) during sub-acute exposure to Butachlor

Document Type : Research Paper


1 Faculty of Marine and Oceanic Sciences, Mazandaran University.

2 Department of Marine Biology, Faculty of Marine and Oceanic Sciences, University of Mazandaran, Babolsar, Iran.

3 Young Researchers and Elite Club, Rasht Branch, Islamic Azad University, Rasht, Iran


Histological changes important for evaluating the effects of materials and chemical contaminants on organisms. This study revealed the histopathological changes in muscle of the goldfish in exposure to Butachlor. For this purpose adult fish were prepared from Aquaculture Center. The samples were distributed randomly in 4 glass aquaria (70 L) with stocking density of 6 fish for each. The aquaria were exposed to the Butachlor with 0.1, 0.14, 0.28 mL/L and the control (without pollutant) for two weeks. At end of the expose period, muscle below the dorsal fin were removed and fixed by Bouin’s solution, processed, embedded in paraffin and sectioned at 6µ thickness and then stained by Hematoxylin-Eosin method. Based on the histological study, changes in striation, nuclear changes, cloudy swelling, hyaline degeneration, granular degeneration and necrosis were observed. Most damage was observed at 0.28 mL/L.