Microalgae lipid is used as a feedstock for biodiesel production. Lipid content is correlated with the microalgae species and growth conditions. In this study, variations in total lipid content in the green microalgae of Chlorella sp. under nitrogen starvation stress after 4, 8 and 12 days was examined. Microalgae were cultured in the Zinder-8+N medium. When they reached the semi-logarithmic growth phase, biomass was harvested and transferred to Zinder-8-N medium. The total lipid content was calculated after extraction with a solution of chloroform/methanol (2:1). Triacylglycerol which is used in biodiesel production separated qualitatively using thin layer chromatography. The results showed that the total lipid content under stress condition after 4, 8 and 12 days increased significantly compared to control (P≤0.05), in average 23.46% more than the control. Also, according to the result of thin layer chromatography, triacylglycerol concentration increased in the microalgae under the stress. In general, nitrogen starvation stress led to increased total lipid content in the green microalgae of Chlorella sp.
Moradi, F., sarmad, J., Ghafoori, H., & Naeemi, A. S. (2015). Total lipids content in the green microalgae of Chlorella sp. under nitrogen starvation stress. Aquatic Physiology and Biotechnology, 3(1), 1-13.
MLA
Fatemeh Moradi; Jannat sarmad; Hossein Ghafoori; Akram sadat Naeemi. "Total lipids content in the green microalgae of Chlorella sp. under nitrogen starvation stress". Aquatic Physiology and Biotechnology, 3, 1, 2015, 1-13.
HARVARD
Moradi, F., sarmad, J., Ghafoori, H., Naeemi, A. S. (2015). 'Total lipids content in the green microalgae of Chlorella sp. under nitrogen starvation stress', Aquatic Physiology and Biotechnology, 3(1), pp. 1-13.
VANCOUVER
Moradi, F., sarmad, J., Ghafoori, H., Naeemi, A. S. Total lipids content in the green microalgae of Chlorella sp. under nitrogen starvation stress. Aquatic Physiology and Biotechnology, 2015; 3(1): 1-13.
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