Document Type : Research Paper
University of Guillan
The goldfish (Carassius auratus auratus) is a freshwater species that is one of the earliest fish to be domesticated, and is one of the most common aquarium fish. To study plasma levels of induced vitellogenin by endocrine disrupting chemicals (EDCs), 17β-estradiol, Bisphenol A, Naphthalene and Butachlor were used. This study was conducted in two separate parts. First, Bisphenol A, Naphthalene and Butachlor were added to aquariums containing goldfish with doses of 500µg/L, 200µg/L and 0.28µg/L, respectively, for 15 days. Then blood sampling was performed on days 5, 10 and 15 of the experiment. Second, 17β-estradiol, Bisphenol A, Naphthalene and Butachlor were injected directly to the fishes with doses of 0.5mg/mL, 50mg/Kg, 50mg/Kg, 20µL and blood sampling was performed after 48h. After separating plasma, phosphate and vitellogenin amounts of each treatment were measured and calculated by using of ALP test. Results from the first experiment showed that there was significant difference between vitellogenin content of treatments and control (P<0.05). The highest level of vitellogenin was devoted to 17β-estradiol and Bisphenol A, respectively. There was no statistically significant difference between Naphthalene and Butachlor treatments. Results from the second experiment showed that there was significant difference between control and treatments (P<0.05), while there was no significant difference between Bisphenol A and Naphthalene treatments (P>0.05). According to the results, in this study, Bisphenol A, Naphthalene and Butachlor can be considered as EDC in both experiments by inducing of vitellogenin synthesis.