Document Type : Research Paper
Chaperones are a group of proteins responsible for controlling proper protein folding in the cell. Heat shock proteins are a group of chaperones, and heat shock protein 90 (Hsp90) are one of the most important members of them as they are the most abundant protein found in a cell (1-2% of total protein). Hsp90 is also responsible for other cellular tasks such as cell signaling. In the present study, research was conducted on Hsp90 from Rutilus frisii kutum. Since there were no data available regarding the sequence of the desired gene, nucleotide sequence analysis of this protein was carried out using PCR technique after cDNA synthesis by using of RNA extracted from the fish liver tissue. The sequence showed high homology with some species such as Cyprinus carpio and Gobiocypris rarus (97% of sequence similarity). Data also showed that the desired protein has 727 amino acids and the gene encoding the protein includes 2181 nucleotides.