sox9 gene expression during stages of larval development and gonadal maturation in the beluga (Huso huso)

Document Type : Research Paper

Authors

1 Assistant Professor in Department of Genetics, International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran.

2 Professor in Iranian Fisheries Research Organization, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.

3 Assistant Professor in Department of Physiology and Biochemistry, International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

4 M.Sc. in Natural Resources Engineering, Department of Physiology and Biochemistry, International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran.

5 M.Sc. in Natural Resources Engineering, Department of Genetics, International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran.

6 Assistant Professor in Department of Physiology and Biochemistry, International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran.

Abstract

sox9 is a protein coding gene that plays a crucial role in the regulation of several cellular activities among vertebrates. The present study was developed for the identification of sox9 gene sequence and expression pattern in different stages of larval and gonadal development of male beluga as it is known a major species in acipenser culture. The results showed that the sox9 sequence in the beluga has high homology with the sox9 sequence in other vertebrates, which represents its fundamental role. Indeed, the study of sox9 gene expression during larval development (1, 3, 6, 15 and 50 days post hatching- dph) revealed that sox9 mRNA is expressed at different larval stages most importantly at 15 dph. However, in differentiated gonads (developmental stages 1-4), sox9 mRNA expressed only at stage 4. In conclusion, it seems that the sox9 expression was related with cartilage formation and organogenesis at early life stages and with testicular development at advanced stages of sexual maturation.

Keywords

References

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Aquatic Physiology and Biotechnology
Volume 5, Issue 2
September 2017
Pages 83-105

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