Chromosomal location of HindIII Satellite DNA in Acipenser persicus and Acipenser gueldenstaedtii by using of fluorescent in situ hybridization (FISH)

Document Type : Research Paper

Authors

1 Assistant Professor in Genetic and Biotechnology Department, International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

2 Professor in Biotechnology Department, Cellular and Molecular Biology Research Center, Shahid Beheshti Medical Sciences University, Tehran, Iran

3 Ph.D. Student in Biochemistry, Biochemistry Department, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

4 M.Sc. in Cell and Molecular Biology, Royan Institute, Tehran, Iran

5 Professor in Iranian Fisheries Research Organization, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

6 Scientific Member in Genetic and Biotechnology Department, International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

Abstract

In this study, a HindIII Satellite DNA (168bp) was isolated from Russian sturgeon, Acipenser gueldenstaedtii and was used as a probe for fluorescent in situ hybridization (FISH) with the chromosomes of Persian sturgeon, Acipenser persicus and Russian sturgeon. After obtaining suitable chromosomal preparations from the fishes through leukocyte culture and labeling the probe with Spectrum orange (Orange-dUTP) through Nick translation method, the probe was hybridized with chromosomes of the fishes on the microscopic slide. In the studied chromosomal preparations, the hybridization colored signals were clearly visible. Counting the produced signals in twelve chromosomal preparations of three fishes from each species showed that the mean number of the signals in the chromosomal preparations of the Persian sturgeon and Russian sturgeon were 66±4 and 68±3 respectively. In this study, accurate kind determining of chromosomes and HindIII SatDNA site on the chromosomes were impossible due to the presence of microchromosomes and heterogeneity of shapes and sizes of colored signals.

Keywords

Aquatic Physiology and Biotechnology
Volume 6, Issue 2
September 2018
Pages 115-134

Files

Share

How to cite

Statistics