Production of polyhydroxybutyrate by cyanobacteria Spirulina sp. under concentrations of mineral salts stress

Document Type : Research Paper

Authors

1 M.Sc. Student in Plant Physiology, Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran

2 Associate Professor in Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran/Associate Professor in Department of Marine Sciences, Caspian Sea Basin Research Center, University of Guilan, Rasht, Iran

3 Ph.D. Student in Biochemistry, Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran

Abstract

Today, due to the high consumption of biodegradable resistant polymers, there is a huge problem of the accumulation of polymer waste in nature. Polyhydroxyalkonoates are biodegradable compounds that are very much considered for replacement with synthetic plastics. In this study, the effects of salt concentration changes on the production of polyhydroxybutyrate by cyanobacteria Spirulina sp. were investigated. Spirulina was cultured in Zarrouk medium containing half (1/2X), equal (X, as control), double (2X) and triple (3X) salt concentrations for 20 days. Quality and quantity of polyhydroxybutyrate were analyzed by measuring growth, protein content and lipid peroxidation. The growth rate of Spirulina sp. in the medium containing increased and decreased concentrations of salts did not change significantly. However, lipid peroxidation and protein increased and decreased, respectively, by increasing salt concentration to 3 times as compared to control. The amount of polyhydroxybutyrate under salt concentrations of 1/2X, X, 2X and 3X were 0.011, 0.014, 0.019 and 0.006 (g/g dcw) respectively. As a result, treatment with 2X salt concentration is more suitable for polyhydroxybutyrate producing than other treatments.

Keywords

References

رستم‌زاد آ. رضایی ح. و هوشمندفر ر. 1396. جداسازی باکتری‌های تولید کننده پلاستیک زیست‌تخریب‌پذیر از خاک‌های آلوده به پساب کارخانه شیر ایلام. مجله علمی پژوهشی دانشگاه علوم پزشکی ایلام، 3(1): 137-125.
Ansari S. and Fatma T. 2016. Cyanobacterial polyhydroxybutyrate (PHB): Screening, optimization and characterization. PLOS ONE, 11(6): 1–20.
Ataei S.A., Vasheghani Farahani E., Tehrani H.A. and Shojaosadati S.A. 2008. Isolation of PHA producing bacteria from date syrup waste. Macromolecular Symposia, 269(1): 11–16.
Balaji S., Gopi K. and Muthuvelan B.A. 2013. Review on production of poly β hydroxybutyrates from cyanobacteria for the production of bio plastics. Algal Research, 2(3): 278–285.
Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram of protein utilizing of protein- day binding. Analytical Biochemistry, 72(1-2): 248–254.
Brandl H., Gross C.A.N., Lenz R.W. and Fuller R.C. 1990. Plastics from bacteria and for bacteria: Poly (hydroxybutyrate) as natural biodegradable polyesters. Advances in Biochemical Engineering/Biotechnology, 41: 77–93.
Brandl H., Gross R.A., Lenz R.W., Fuller R.C. 1988. Pseudomonas oleovorans as a source of poly betahydroxyalkanoates for potential applications as biodegradable polyesters. Applied and Environmental Microbiology, 54(8): 1977–1982.
Campbell J., Stevens J.S. and Balkwill D.L. 1982. Accumulation of poly-β hydroxybutyrate in Spirulina plantensis. Journal of Bacteriology, 149: 361–363.
Capone D.G., Burns J.A., Montoya J.P., Subramaniam A., Mahaffey A.C., Gunderson T., Michaels A.F. and Carpenter E.J. 2005. Nitrogen fixation by Trichodesmium spp.: An important source of new nitrogen to the tropical and subtropical North Atlantic Ocean. Global Biogeochemical Cycles, 19(2): 1–17.
Chua H., Hu W.F. and Ho. L.Y. 1997. Recovery of biodegradable polymers from food-processing wastewater activated sludge system. Journal-Institution of Engineers Singapore, 37: 9–14.
Deschoenmaeker F., Facchini R., Carlos J., Pino C., Bayon-Vicente G., Sachdeva N., Flammang P. and Wattiez R. 2016. Nitrogen depletion in Arthrospira sp. PCC 8005, an ultrastructural point of view. Journal of Structural Biology, 196: 385–393.
Drosg B., Fritz I., Gattermayr F. and Silvestrini L. 2015. Photo-autotrophic production of poly (hydroxyalkanoates) in cyanobacteria. Chemical and Biochemical Engineering Quarterly, 29(2): 145–156.
Fernandez D., Rodriguez E., Bassas M., Vinas M., Solanas A.M., Llorens J., Marques A.M. and Manresa A. 2005. Agro-industrial oily wastes as substrates for PHA production by the new strain Pseudomonas aeruginosa NCIB 40045: Effect of culture conditions. Biochemical Engineering Journal, 26: 159–167.
Gao Y., Cui Y., Xiong W., Li X. and Wu Q. 2009. Effect of UV-C on algal evolution and differences in growth rate, pigmentation and photosynthesis between prokaryotic and eukaryotic algae. Photochemistry and Photobiology, 85: 774–782.
 Griffin G. 1994. Chemistry and Technology of Biodegradable Polymers. Springer, Netherlands. 154P.
Heath R.L. and Packer L. 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125(1): 189–198.
Hong K., Sun S., Tian W., Chen G. and Huang W. 1999. A rapid method for detecting bacterial polyhydroxyalkanoates in intact cells by Fourier transform infrared spectroscopy. Applied Microbiology and Biotechnology, 51(4): 523–526.
Ismaiel M.M., MahmoudEl-Ayouty Y. and Piercey-Normore M. 2016. Role of pH on antioxidants production by Spirulina (Arthrospira) platensis. Brazilian Journal of Microbiology, 47(2): 298–304.
Jau M.H., Yew S.P., Toh P.S.Y., Chong A.S.C., Chu W.L. and Phang S.M. 2005. Biosynthesis and mobilization of poly(3-hydroxybutyrate) [P(3HB)] by Spirulina platensis. International Journal of Biological Macromolecules, 36(3): 144–151.
Kansiz M., Billman-Jacobe H. and McNaughton D. 2000. Quantitative determination of the biodegradable polymer poly (beta-hydroxybutyrate) in a recombinant Escherichia coli strain by use of mid-infrared spectroscopy and multivariative statistics. Applied and Environmental Microbiology, 66(8): 3415–3420.
Kumagai Y. and Doi Y. 1992. Enzymatic degradation of binary blends of microbial poly (3-hydroxy butyrate) with enzymatically active polymers. Polymer Degradation and Stability, 37(3): 253–256.
Lam M.K. and Lee K.T. 2012. Microalgae biofuels: A critical review of issues, problems and the way forward. Biotechnology Advances, 30(3): 673–690.
Lee C., Kim J., Do H. and Hwang S. 2008. Monitoring thiocyanate-degrading microbial community in relation to changes in process performance in mixed culture systems near washout. Water Research, 42(4-5): 1254–1262.
Li Z.Y., Guo S.Y., Li L. and Cai M.Y. 2007. Effects of electromagnetic field on the batch cultivation and nutritional composition of Spirulina platensis in an air-lift photobioreactor. Bioresource Technology, 98(3): 700–705.
Luef K.P., Stelzer F. and Wiesbrock F. 2015.  Poly(hydroxyalkanoate)s in medical applications. Chemical and Biochemical Engineering, 29(2): 287–297.
Nishioka M., Nakai K., Miyake M., Asada Y. and Taya M. 2001. Production of poly-b hydroyxybutyrate by thermophilic cyanobacterium, Synechococcus sp. MA19 under phosphate limitation. Biotechnology Letters, 23: 1095–1099.
Paerl H.W. 2012. Marine plankton. P: 127–153. In: Whitton B.A. (Ed.). Ecology of Cyanobacteria II. Springer, Netherlands.
Panda B., Sharma L. and Mallick N. 2005. Poly-b-hydroxybutyrate accumulation in Nostoc muscorum and Spirulina platensis under phosphate limitation. Journal Plant Physiology, 162: 1376–1379.
Pinto E., Sigaud-Kutner T., Leitao M. and Okamoto O. 2003. Heavymetal induced oxidative stress in algae. Journal of Phycology, 39(6): 1008–1018.
Rippka R., Neilson A., Kunica W.A.R. and Cohenbazire G. 1971. Nitrogen fixation by unicellular blue-green algae. Archiv fur Mikrobiologie, 76: 341–348.
Sayaka H., Masatoshi T., Takashi O., Mami M., Tomohisa H., Akio T., Yoichi N., Shigeru C., Morifumi H. and Munehiko A. 2015. Genetic engineering and metabolite profiling for overproduction of polyhydroxybutyrate in cyanobacteria. Journal of Bioscience and Bioengineering, 120(5): 510–517.
Sharma L. and Mallick N. 2005. Accumulation of poly-β-hydroxybutyrate in Nostoc muscorum: Regulation by pH, light-dark cycles, N and P status and carbon sources. Bioresourse Technology, 96: 1304–1310.
Shetty P., Shenai P. and Chatra L. 2013. Efficacy of Spirulina as an antioxidant adjuvant to corticosteroid injection in management of oral submucous fibrosis. Indian Journal of Dental Research, 24(3): 347–350.
Shi J., Votruba A.R., Farokhzad O.C. and Langer R. 2010. Nanotechnology in drug delivery and tissue engineering: From discovery to applications. Nano Letters, 10(9): 3223–3230.
Shrivastav A., Kim H.Y. and Kim Y.R. 2013. Advances in the applications of polyhydroxyalkanoate nanoparticles for novel drug delivery system. Nanobiotechnology, 1: 1–12. 
Shrivastav A., Mishra S.K., Mishra S. 2010. Polyhydroxyalkanoate (PHA) synthesis by Spirulina subsalsa from Gujarat coast of India. International Journal of Biological Macromolecules, 46(2): 255–260.
Singh P. and Parmar N. 2011. Isolation and characterization of tow novel polyhydroxybutyrate producing bacteria. African Journal of Biotechnology, 10(24): 4907–4919.
Singh S.C., Sinha R.P. and Hader D.P. 2002. Role of light and fatty acids in stress tolerance in cyanobacteria. Acta Protozoologica, 41(4): 297–308.
Zarrouk C. 1966. Contribution to the Cyanophyceae study: Influence various physical and chemical factors on growth and photosynthesis of Spirulina maxima (Setch et Gardner) Geither extract. Ph.D. Thesis, University of Paris-Saclay, France. 146P.
Zhang Y.M., Chen H., He C.L. and Wang Q. 2013. Nitrogen starvation induced oxidative stress in an oil-producing green alga Chlorella sorokiniana C3. PLOS ONE, 8(7): 1–12.
Aquatic Physiology and Biotechnology
Volume 8, Issue 1
June 2020
Pages 165-187

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