Modulation of growth performance, digestive enzyme activity, serum biochemical and immunological parameters in common carp (Cyprinus carpio) using probiotic Bacillus cereus

Document Type : Research Paper

Authors

1 M.Sc. Student in Fisheries, Department of Fisheries, Faculty of Natural Resources, University of Zabol, Zabol, Iran

2 Assistant Professor in Department of Fisheries, Faculty of Natural Resources, University of Zabol, Zabol, Iran

3 Assistant Professor in Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

4 Associate Professor in Department of Food Hygiene and Aquatic Animals, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

Abstract

The genus of Bacillus, due to its higher resistance to harsh environmental conditions, is the most broadly used probiotic in aquaculture. Present study was designed to investigate the effects of Bacillus cereus on growth, digestive enzyme activity and serum biochemical and immunological indices in common carp (Cyprinus carpio). After the 14-day adaptation, fish with mean weight of 70.57±0.72g were allocated into 12 tanks (15 fish per tank) and fed basal diet (control group) and diets containing probiotic B. cereus in three doses, including 107, 108, and 109 CFU.g-1. After 45 days feeding, diet supplemented with higher dose of probiotic showed significantly better result of weight gain, digestive enzyme activities for trypsin, amylase and lipase, mean white blood cell and lymphocyte counts, total antibody level, lysozyme and protease activities than those with the control diet (p < 0.05). Meanwhile, albumin, triglyceride and aspartate aminotransferase levels were remarkably higher in medium and high doses than the control group (p < 0.05). Based on the present results, supplementation of probiotic B. cereus, especially at 109 CFU.g-1, is recommended to enhance the growth performance, hemato-immunological responses, and digestive enzyme activities in common carp.

Keywords

References

کلیائی ز.، آبرومند ع. و ضیایی‌نژاد س. 1395. تاثیر باکتری زیست‌یار Bacillus subtilisمستخرج از روده ماهی کپور معمولی (Cyprinus carpio) بر عملکرد رشد و بقاء آن. نشریه توسعه آبزی‌پروری، 10(2): 108-99.
Ahmadifar E., Sadegh T.H., Dawood M.A.O., Dadar M. and Sheikhzadeh N. 2020. The effects of dietary Pediococcus pentosaceus on growth performance, hemato-immunological parameters and digestive enzyme activities of common carp (Cyprinus carpio). Aquaculture, 516: 1–39 (734656).
Andani H., Tukmechi A., Meshkini S. and Sheikhzadeh N. 2012. Antagonistic activity of two potential probiotic bacteria from fish intestines and investigation of their effects on growth performance and immune response in rainbow trout (Oncorhynchus mykiss). Journal of Applied Ichthyology, 28: 728–734.
Bandyopadhyay P. and Das Mohapatra P.K. 2009. Effect of a probiotic bacterium Bacillus circulans PB7 in the formulated diets: On growth, nutritional quality and immunity of Catla catla (Ham). Fish Physiology and Biochemistry, 35: 467–478.
Bernfeld P. 1955. Amylase, ɑ and β. P: 149–158. In Colowick S.P. and Kaplan N.O. (Eds.). Methods in Enzymology. Academic Press, USA.
Blaxhall P.C. and Daisley K.W. 1973. Routine haematological methods for use with fish blood. Journal of Fish Biology, 5: 771–781.
Bogut I., Milakovic Z., Bukvic Z., Brkic S. and Zimmer R. 1999. Influence of probiotic Streptococcus faecium on growth and content of intestinal microflora in carp (Cyprinus carpio). Czech Journal of Animal Science, 43(5): 231–235.
Boshra H., Li J. and Sunyer J.O. 2006. Recent advances on the complement system of teleost fish. Fish and Shellfish Immunology, 20: 239–262.
Bulow L. and Mosbach K. 1987. The expression in E. coli of a polymeric gene coding for an esterase mimic catalyzing the hydrolysis of p-nitrophenyl esters. FEBS Letters, 210(2): 147–152.
Burgos-Aceves M.A., Lionetti L. and Faggio C. 2019. Multidisciplinary haematology as prognostic device in environmental and xenobiotic stress-induced response in fish. Science Total Environment, 670: 1170–1183.
Cho H.C. and Lee S.M. 2012. Onion powder in the diet of the Olive flounder (Paralichthys olivaceus): Effects on the growth, body composition and lysozyme activity. World Aquaculture Society, 43(1): 30–38.
Dawood M.A.O., Koshio S. and Esteban M.A. 2018. Beneficial roles of feed additives as immunostimulants in aquaculture: A review. Reviews in Aquaculture, 10: 950–974.
De Souza D.M., Bernardes Martins G., Noguez Piedras S.R., Fernandes Pouey J.L.O., Robaldo R.B. and Leivas Leite F.P. 2012. Probiotic actions of Bacillus cereus var. toyoi and Saccharomyces boulardii in silver catfish (Rhamdia quelen) larvae culture. Revista Brasileira de Zootecnia, 41(3): 815–819.
Demers N.E. and Bayne C.J. 1997. The immediate effects of stress on hormones and plasma lysozyme in rainbow trout. Developmental and Comparative Immunology, 21: 363–373.
Evelyn E. and Silva F.V.M. 2015. Thermosonication versus thermal processing of skim milk and beef slurry modeling the inactivation kinetics of psychrotrophic Bacillus cereus spores. Food Research International, 67: 67–74.
Hao K., Liu J.Y., Ling F., Liu X.L., Lu L., Xia L. and Wang G.X. 2014. Effects of dietary administration of Shewanella haliotis D4, Bacillus cereus D7 and Aeromonas bivalvium D15, single or combined, on the growth, innate immunity and disease resistance of shrimp, Litopenaeus vannamei. Aquaculture, 428-429: 141–149.
Hidalgo M.C., Urea A. and Sanz A. 1999. Comparative study of digestive enzymes in fish with different nutritional habits. proteolytic and amylase activities. Aquaculture, 170: 267–283.
Hoseinifar S.H., Zoheiri F. and Lazado C.C. 2016. Dietary phyto immunostimulant Persian hogweed (Heracleum persicum) has more remarkable impacts on skin mucus than on serum in common carp (Cyprinus carpio). Fish and Shellfish Immunology, 32: 59–77.
Joborn A., Olsson C., Westerdahl A., Conway P.L. and Kjellberg S. 1997. Colonization in the fish intestinal tract and production of inhibitory substances in intestinal mucus and faecal extract by Camobacterium sp. Strain. Journal of Fish Disease, 20: 383–392.
Khan A., Shah N., Sayed Khan M., Saleem Ahmad M., Farooq M., Adnan M., Muhammad Jawad S., Ullah H. and Muhammad Yousafzai A. 2016. Quantitative determination of lethal concentration LC50 of atrazine on biochemical parameters; total protein and serum albumin of freshwater fish grass carp (Ctenopharyngodon idella). Polish Journal of Environmental Studies, 25(4): 1555–1561.
Lopez-Vasquez K., Castro-Perez C.A. and Val A.L. 2009. Digestive enzymes of eight Amazonian teleosts with different feeding habits. Journal of Fish Biology, 74: 1620–1628.
Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J. 1951. Protein measurement with the Folin phenol reagent. Journal of Biology and Chemistry, 193: 265–275.
Merrifield D., Bradley G., Harper G., Baker R., Munn C. and Davies S. 2011. Assessment of the effects of vegetative and lyophilized Pediococcus acidilactici on growth, feed utilization, intestinal colonization and health parameters of rainbow trout (Oncorhynchus mykiss Walbaum). Aquaculture Nutrition, 17: 73–79.
Messaoudi K., Clavel T., Schmitt P.H. and Duport C. 2010. Mediates carbohydrate-dependent regulation of catabolic and enterotoxin genes in Bacillus cereus F73/4430. Research in Microbiology, 61(1): 30–39.
NavinChandran M., Iyapparaj P., Moovendhan S., Ramasubburayan R., Prakash S., Immanuel G. and Palavesam A. 2014. Influence of probiotic bacterium Bacillus cereus isolated from the gut of wild shrimp Penaeus monodon in turn as a potent growth promoter and immune enhancer in P. monodon. Fish and Shellfish Immunology, 36: 38–45.
Nayak S.K. 2010. Probiotics and immunity: A fish perspective. Fish and Shellfish Immunology, 29: 2–14.
Nayak S.K., Swain P. and Mukherjee S.C. 2007. Effect of dietary supplementation of probiotic and vitamin C on the immune response of Indian major carp, Labeo rohita (Ham.). Fish and Shellfish Immunology, 23: 892–896.
Otatake M., Kiryu I. and Nakanishi T. 2002. Development of vaccine delivery method for fish: Parcutaneous administration by immersion with application of multiple puncture instruments. Vaccine, 1: 3764–3769.
Perez-Sanchez T., Ruiz-Zarzuela I., De Blas I. and Balcazar J.L. 2014. Probiotics in aquaculture: A current assessment. Reviews in Aquaculture, 6: 133–146.
Ringo E., Olsen R.E., Vecino J.L.G., Wadsworth S. and Song S.K. 2012. Use of immunostimulants and nucleotides in aquaculture: A review. Journal of Marine Science: Research and Development, 2(1): 1–22.
Sheikhzadeh N., Tayefi-Nasrabadi H., Khani Oushan A. and Najafi Enferadi M.H. 2012. Effects of Haematococcus pluvialis supplementation on antioxidant system and metabolism in rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry, 38(2): 413–419.
Siwicki A.K., Anderson D.P. and Rumsey G.L. 1994. Dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against furunculosis. Veterinary Immunology and Immunopathology, 41: 125–139.
Song S.K., Bo R.B., Kim D., Park J., Jungjoon K., Hyun D.K. and Ringo E. 2014. Prebiotics as immunostimulants in aquaculture: A review. Fish and Shellfish Immunology, 40: 40–48.
Verschuere L., Rombaut G., Sorgeloos P. and Verstraete W. 2000. Probiotic bacteria as biological control agents in aquaculture. Microbiology and Molecular Biology Reviews, 64(4): 655–671.
Wang Y.B. and Xu Z. 2006. Effect of probiotics for common carp (Cyprinus carpio) based on growth performance and digestive enzyme activities. Animal Feed Science and Technology, 127: 283–292.
Wu Z.X., Feng X., Xie L.L., Peng X.Y., Yuan J. and Chen X.X. 2012. Effect of probiotic Bacillus subtilis Ch9 for grass carp, Ctenopharyngodon idella (Valenciennes, 1844), on growth performance, digestive enzyme activities and intestinal microflora. Journal of Applied Ichthyology, 28: 721–727.
Yeganeh S., Teimouri M. and Keramat Amirkolaie A. 2015. Dietary effects of Spirulina platensison hematological and serum biochemical parameters of rainbow trout (Oncorhynchus mykiss). Research in Veterinary Science, 101: 84–88.
Zhao Y., Yuan L., Wan J., Sun Z., Wang Y. and Sun H. 2016. Effects of potential probiotic Bacillus cereus EN25 on growth, immunity and disease resistance of juvenile sea cucumber Apostichopus japonicas. Fish and Shellfish Immunology, 49: 237–242.
Aquatic Physiology and Biotechnology
Volume 8, Issue 8
December 2020
Pages 1-19

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