Extraction and characterization of UV-absorbing compounds from sea urchin Echinometra mathaei

Document Type : Research Paper

Authors

1 Ph.D. Student in Marine Biology, Department of Marine Biology, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran

2 AAssociate Professor in Department of Marine Biology, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran

3 Assistant Professor in Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran

4 Associate Professor in Department of Chemistry, Faculty of Sciences, University of Hormozgan, Bandar Abbas, Iran

Abstract

In this study, the occurrence of mycosporin-like amino acids in the sea urchin Echinometra mathaei from Persian Gulf was assessed for the first time. Mycosporine-like amino acids (MAAs) are UV-absorbing compounds that are synthesized by some organisms to counteract the harmful effects of UV radiation. Mycosporine-like amino acids in different parts of E. mathaei was assessed by HPLC and LC-MS analysis. Moreover, antioxidant ability of total extract of shell as well as its fractions were evaluate using DPPH assay. Combination of UV and LC-MS data values revealed the occurrence of Shinorine in the shell of E. mathaei. Total extract of urchin shell showed IC50 value of 231µg/mL which contributed to the mycosporine-like amino acid shinorine.

Keywords

References

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Aquatic Physiology and Biotechnology
Volume 8, Issue 8
December 2020
Pages 99-115

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