The effect of oil-emulsified Ovaprim™ on spawning induction and artificial reproduction of bream (Abramis brama) female broodstock

Document Type : Research Paper

Authors

1 Ph.D. Student in Fisheries, Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran

2 M.Sc. Student in Fisheries, Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran

3 Professor in Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran

Abstract

The present study was carried out to determine the effect of emulsified OvaprimTM with almond oil on spawning induction of Abramis brama, as well as, the spermatological parameters of males. In the first (C), second (OO) and third (OS) treatments, females were injected with 1mL.kg-1 body weight (BW) of bitter almond oil, 1mL.kg-1 BW OvaprimTM with almond oil (1:1) and 1mL.kg-1 BW OvaprimTM with 0.9% NaCl solution (1:1), respectively. In each treatment, 10 females (weight range between 802 to 934g) were injected. Moreover, 26 males were injected in two replicates with 0.6mL.kg-1 BW by OvaprimTM with 0.9% NaCl solution (1:2). Based on the results, the highest ovulation rate was observed in OS treatment (100%) and the lowest ovulation rate was observed in control group (0%) (P<0.05). Moreover, there was a significant difference between OO (722.5±68.4 degree-hour) and OS (578.0±44.2 degree-hour) treatments in latency time (P<0.05). The significant difference was observed between control group (0g) and OO (52.4±34.6g) and OS (72.3±0.2g) treatments in average weight of obtained eggs (P<0.05). The significant differences were not found between OO and OS treatments in absolute fecundity, relative fecundity, the average number of eggs per gram, fertilization rate, and incubation period (P>0.05). Based on the results of this study, the mean of sperm volume, sperm activity, motility time, spermatocrit value, and spermatozoa concentration of Abramis brama were 13.6±5.9mL, 56.7±12.1%, 95.2±17.6s, 30.0±8.8%, 11.0±3.3×109mL-1, respectively. According to the results, it is recommended that the emulsified OvaprimTM with oil can be used to induce spawning of bream in hatcheries.

Keywords

References

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Aquatic Physiology and Biotechnology
Volume 8, Issue 4
March 2021
Pages 1-18

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