Investigation of antibacterial effect of red algae Gracilaria gracilis extract

Document Type : Research Paper

Authors

1 M.Sc. in Biotechnology, Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran

2 Assistant Professor in Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran

3 M.Sc. in Plant Physiology, Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran

Abstract

Today, due to increase in resistance of antibiotics, it is necessary to find suitable alternatives to natural materials. Algae also have many applications in medicine, pharmacy and the food industry.  Therefore, the present study was performed to investigate the antibacterial activity of red algae Gracilaria gracilis extract. In this study, the active compounds of methanolic extract of red algae G. gracilis were identified by gas chromatography-mass spectrometry (GC/MS). The Antimicrobial effect of algae extract on bacteria were investigated by sequential dilution method. The chemical analysis of the algae extract compounds showed that the major components of the extract were phenylethine, silacyclopntenen, phthalic acid, n-hexadecanoic acid, dibutyl phthalate, phytol and gibberellin. In the study of minimum inhibitory concentration and minimum bactericidal concentration, it was shown that Pseudomonas aeruginosa and Staphylococcus aureus with algae extract with a minimum inhibitory concentration of 6.2 and 112.5 mg/mL, respectively, compared to other studied bacteria, showed more sensitivity. Based on the results, the methanolic extract of red algae G. gracilis had good antimicrobial activity. Therefore, clinical research is necessary for the clinical applications of this extract.

Keywords

References

ببری ش.، دوستی م.ح.، فاتحی ل. و سالاری ع.ا. 1391. تاثیر گیاه تشنه‌داری (Scrophularia striata) بر روی رفتارهای اضطرابی و افسردگی در موش‌های سوری نر بالغ. مجله علوم دارویی، 18(2): 140-133.
پیمانی ج.، قرایی ا.، غفاری م. و طاهری ع. 1393. بررسی اثرات ضدباکتریایی و ضدقارچی جلبک دریایی (Gracilaria arcuata) از سواحل چابهار. مجله دانشگاه علوم پزشکی قم، 8(1): 75-69.
حاجی مهدی‌پور ه.، خانوی م.، شکرچی م.، عابدی ز. و پیرعلی همدانی م. 1388. بررسی بهترین روش استخراج ترکیبات فنلی موجود در گیاه سرخارگل. فصلنامه گیاهان دارویی، 4(32): 152-145.
دوستی ب. 1398. مقایسه اثرات ضدباکتریایی و ضدقارچی صمغ گیاه بنه (Pistacia atlantica) با برخی آنتی‌بیوتیک‌های رایج درمانی. مجله پژوهش‌های سلولی مولکولی (زیست‌شناسی ایران)، 32(2): 124-115.
قرنجیک ب.م. و روحانی قادیکلاییک. 1388. اطلس جلبک‌های دریایی سواحل خلیج فارس و دریایی عمان. انتشارات موسسه تحقیقات شیلات. 313ص.
Abbot I.A. and Hollenberg G.J. 1976. Marine Algae of California. Stanford University Press, USA. 827P.
Albuquerque M.R., Takaki C. and Koening M.L. 1983. Detection of antimicrobial activity in marine seaweeds. Revista do Instituto de Antibiotics, Universidade Federal de Pernambuco, 21: 127–138.
Aparna V., Dileep K.V., Mandal P.K., Karthe P., Sadasivan C. and Haridas M. 2012. Anti-inflammatory property of n-hexadecanoic acid: Structuralevidence and kinetic assessment. Chemical Biology and Drug Design, 80(3): 434–439.
Barsanti L. and Gualtieri P. 2006. Algae Anatomy, Biochemistry and Biotechnology. Taylor and Francis Group, USA. 344P.
Bouhla R., Riadi H., Martínez J. and Bourgougnon N. 2010. The antibacterial potential of the seaweeds (Rhodophyceae) of the Strait of Gibraltar and the Mediterranean Coast of Morocco. African Journal of Biotechnology, 9(38): 6365–6372.
Cyriac B. and Eswaran K. 2015. GC-MS determination of bioactive components of Gracilaria dura (C. Agardh) Agardh J. Science Research Reporter, 5(2): 100–105.
Desbois A.P. and Smith V.J. 2010. Antibacterial free fatty acids: Activities, mechanisms of action and biotechnological potential. Applied Microbiology and Biotechnology, 85(6): 1629–1642.
Forbes B.A., Sahm D.F., Weissfeld A.S. and Trevino E.A. 2013. Methods for testing antimicrobial effectiveness. P: 171–94. In: Baron E.J., Peterson L.R. and Finegold S.M. (Eds.). Bailey and Scott's Diagnostic Microbiology. Mosby, USA.
Ghaneian M.T., Ehrampoush M.H., Jebali A., Hekmatimoghaddam S.D. and Mahmoudi M. 2015. Antimicrobial  activity,  toxicity and  stability  of  phytol  as  a    novel surface disinfectant. Environmental Health Engineering and Management, 2(1): 13–16.
Gharanjik B.M. and Abkenar A.M. 2000. Identification of seaweed in Sistan and Baluchestan Province costal zone. Iranian Scientific Fisheries Journal, 9(1): 37–49.
Khatiwora E., Vaishali B.A., Kulkarni M., Deshpande N.R. and Kashalkar R.V. 2012. Antibacterial activity of dibutyl phthalate: A secondary metabolite isolated from Ipomoea carnea stem. Journal of Pharmaceutical Research, 5(1):150–152.
Lavanya R. and Veerappan N. 2011. Antibacterial potential of six seaweeds collected from Gulf of Mannar of southeast coast of India. Advances in Biological Research, 5(1): 38–44.
Lee W., Woo E.R. and Lee D.G. 2016. Phytol has antibacterial property by inducing oxidative stress response in Pseudomonas aeruginosa. Free Radical Research, 50(12): 1309–1318.
Mini Shobi T. and Viswanathan M.B. 2018. Antibacterial activity of di-butyl phthalate isolated from Begonia malabarica. Journal of Applied Biotechnology and Bioengineering, 5(2): 97–100.
Prasad M.P., Shekhar S. and Rindhe G. 2014. Antibacterial activity of seaweed (Gracilaria) extracts against Human pathogens. Asian Journal of Biological and Life Sciences, 3: 219–222.
Rhimou B., Hassane R., Jose M. and Nathalie B. 2010. The antibacterial potential of the seaweeds (Rhodophyceae) of the Strait of Gibraltar and the Mediterranean Coast of Morocco. African Journal of Biotechnology, 9(38): 6365–6372.
Roy R.N., Laskar S. and Sen S.K. 2006. Dibutyl phthalate, the bioactive compound produced by Streptomyces albidoflavus. Microbiological Research, 161(2): 121–126.
Silva G.C., Albuquerque-Costa R., Oliveira-Peixoto J.R., Pessoa-Nascimento F.E., Macedo-Carneiro P.B. and Fernandes-Vieira R.H.S. 2013. Tropical Atlantic marine macroalgae with bioactivity against virulent and antibiotic resistant Vibrio. Latin American Journal of Aquatic Research, 41(1): 183–188.
Taskin E. and Ozturk M. 2007. Antibacterial activity of some marine algae from the Aegean Sea (Turkey). African Journal of Biotechnology, 6(24): 2746–2751.
Taskin E., Caki Z., Ozturk M. and Taskin E. 2010. Assessment of in vitro antitumoral and antimicrobial activities of marine algae harvested from the eastern Mediterranean Sea. African Journal of Biotechnology, 9(27): 4272–4277.
Tuney I., Cadirci B.H., Unal D. and Sukatar A. 2006. Antimicrobial activities of the extracts of marine algae from the coast of Urla (Izmir, Turkey). Turkish Journal of Biology, 30: 171–175.
Varier K.M., Milton M.C.J., Arulvasu C. and Gajendran B. 2013. Evaluation of antibacterial properties of selected red seaweeds from Rameshwaram, Tamil Nadu, India. Journal of Academia and Industrial Research, 1(11): 667–670.
Yff B.T., Lindsey K.L., Taylor M.B., Erasmus D.G. and Jager A.K. 2002. The pharmacological screening of Pentanisia prunelloides and the isolation of the antibacterial compound palmitic acid. Journal of Ethnopharmacology, 79(1): 101–107.
Aquatic Physiology and Biotechnology
Volume 8, Issue 4
March 2021
Pages 115-130

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