Investigating verification of sturgeon caviar in cosmetic products using barcoding method of mitochondrial genes

Document Type : Research Paper

Authors

1 Assistant Professor in Department of Genetics and Biotechnology, International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

2 Scientific Member in Department of Genetics and Biotechnology, International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

Abstract

In order to evaluate the presence or absence of DNA in cream and caviar extract samples, four samples of cream and one sample of caviar extract were evaluated for DNA concentration using Nanodrop and agarose gel analysis. The amount of DNA extracted in the samples of cream and caviar extract based on Nanodrop measurement was equal to 5 to 20 ng/µL, but it was not possible to examine these samples on agarose gel due to the little concentration. In order to test for the presence or absence of caviar extract and cream in the cosmetic cream, the mitochondrial DNA tracing method of sturgeon species was used by amplifying important fragments in global barcoding tests such as cytochrome oxidase I (COI) and cytochrome b (Cyt b). Amplified fragments in cream and caviar extract were analyzed by sequencing. The fragment length in COI gene was 650 nucleotides and in Cyt b was 1200 nucleotides. The final results showed that the cream and extract samples contain sturgeon DNA. The sturgeon species used to make the cream was closer to the Siberian sturgeon species (Acipenser baerii).

Keywords

References

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Aquatic Physiology and Biotechnology
Volume 9, Issue 2
September 2021
Pages 1-20

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