Enhancing the antimicrobial effects of gelatin-based composite films extracted from kilka fish waste and xanthan gum using Anghozeh oil

Document Type : Research Paper


1 Assistant Professor in Biology Department, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran

2 Assistant Professor in Department of Microbiology, Hidaj Branch, Islamic Azad University, Hidaj, Iran


Today, the use of natural materials in wound dressing has attracted more attention that can overcome the serious environmental problems caused by conventional synthetic materials. In this study, first gelatin was extracted from Caspian kilka fish waste and then used to make composite films in pure form (G), in combination with xanthan (GX) and Anghouzeh oil (GXA). Mechanical properties, swelling index and surface morphology of the films were investigated. The antibacterial and anti-biofilm effects of G, GX and GXA films were also evaluated. The highest tensile strength was recorded for GX film (37.30±0.59MPa) and the lowest value was recorded for pure gelatin film (11.74±0.2MPa). For elongation at break, the highest value was measured in GXA film (54.87±0.21%) and the lowest was in G film (15.61±0.68%). The highest swelling index was recorded for GX film at 300±15.3%. GXA film presented the highest antimicrobial and anti-biofilm activities. The results of these study showed that the film made of gelatin in combination with xanthan and Anghouzeh oil has ideal mechanical and antibacterial properties to use in wound dressing structure.


Abd Elgadir M., Mirghani M.E. and Adam A. 2013. Fish gelatin and           its applications in selected pharmaceutical aspects as alternative source to pork gelatin. Journal of Food, Agriculture and Environment, 11: 73–79.
Abdollahi M., Damirchi S., Shafafi M., Rezaei M. and Ariaii P. 2019. Carboxymethyl cellulose-agar biocomposite film activated         with summer savory essential                 oil as an antimicrobial agent. International Journal of Biological Macromolecules, 126: 561–568.
Acevedo-Fani A., Salvia-Trujillo         L., Rojas-Grau M.A. and Martin-Bellos O. 2015. Edible films from essential-oil-loaded nanoemulsions: Physicochemical characterization and antimicrobial properties. Food Hydrocolloids, 47: 168–177.
Ahmed J., Mulla M., Joseph A., Ejaz M. and Maniruzzaman M. 2020. Zinc oxide/clove essential oil incorporated type B gelatin nanocomposite formulations: A proof-of-concept study for 3D printing applications. Food Hydrocolloids, 98: 1–7 (105256).
Ahmed J., Mulla M.Z. and Arfat Y.A. 2016. Thermo-mechanical, structural characterization and antibacterial performance of solvent casted polylactide/ cinnamon oil composite films. Food Control, 69: 196–204.
Altay F. and Gunasekaran S. 2013. Gelling properties of gelatin-xanthan gum systems with high levels of co-solutes. Journal of Food Engineering, 118: 289–295.
Alves A.L., Marques A.L., Martins E., Silva T.H. and Reis R.L. 2017. Cosmetic potential of marine fish skin collagen. Cosmetics, 4: 1–16 (39).
Amalraj A., Haponiuk J.T., Thomas S. and Gopi S. 2020. Preparation, characterization and antimicrobial activity of polyvinyl alcohol/gum arabic/chitosan composite films incorporated with black pepper essential oil and ginger essential oil. International Journal of Biological Macromolecules, 151: 366–375.
Augustine R., Kalarikkal N. and Thomas S. 2015. An in vitro method for the determination of microbial barrier property (MBP) of porous polymeric membranes for skin substitute and wound dressing applications. Tissue Engineering and Regenerative Medicine, 12: 12–19.
Bigi A., Cojazzi G., Panzavolta S., Roveri N. and Rubini K. 2002. Stabilization of gelatin films           by crosslinking with genipin. Biomaterials, 23: 4827–4832.
Binsi P., Nayak N., Sarkar P., Joshy C., Ninan G. Ravishankar C. 2017. Gelation and thermal characteristics of microwave extracted fish gelatin–natural gum composite gels. Journal of Food Science and Technology, 54: 518–530.
De Carvalho R. and Grosso C.      2004. Characterization of gelatin based films modified with transglutaminase, glyoxal and formaldehyde. Food Hydrocolloids, 18: 717–726.
Detwiler L.A. and Rubenstein R. 2000. Bovine spongiform encephalopathy: An overview. American Society for Artificial Internal Organs Journal, 46: 73–79.
Divya K., Ramalakshmi K., Murthy P.S. and Rao L.J.M. 2014. Volatile oils from Ferula asafoetida varieties and their antimicrobial activity. LWT-Food Science and Technology, 59: 774–779.
Etxabide A., Vairo C., Santos-Vizcaino E., Guerrero P., Pedraz J.L., Igartua M., De La Caba K. and Hernandez R.M. 2017. Ultra thin hydro-films based on lactose-crosslinked fish gelatin for wound healing applications. International Journal of Pharmaceutics, 530: 455–467.
Fitzpatrick P., Meadows J., Ratcliffe I. and Williams P.A. 2013. Control of the properties of xanthan/glucomannan mixed gels by varying xanthan fine structure. Carbohydrate Polymers, 92: 1018–1025.
Galus S. and Kadzinska J. 2016. Moisture sensitivity, optical, mechanical and structural properties of whey protein-based edible films incorporated with rapeseed oil. Food Technology and Biotechnology, 54: 78–89.
Gomez-Guillen M.C., Turnay J., Fernandez-Diaz M., Ulmo N., Lizarbe M.A. and Montero P. 2002. Structural and physical properties of gelatin extracted  from different marine species:               A comparative study. Food Hydrocolloids, 16: 25–34.
Guerrero P., Stefani P., Ruseckaite R. and De La Caba K. 2011. Functional properties of films based on soy protein isolate and gelatin processed by compression molding. Journal of Food Engineering, 105: 65–72.
Hazirah M.N., Isa M. and Sarbon N. 2016. Effect of xanthan gum on the physical and mechanical properties of gelatin-carboxymethyl cellulose film blends. Food Packaging and Shelf Life, 9: 55–63.
Herrera P., Burghardt R. and Phillips T. 2000. Adsorption of Salmonella enteritidis by cetylpyridinium-exchanged montmorillonite clays. Veterinary Microbiology, 74: 259–272.
Iranshahi M., Fata A., Emami B., Shahri B.M.J. and Bazzaz B.S.F. 2008. In vitro antifungal activity of polysulfides-rich essential oil of Ferula latisecta fruits against human pathogenic dermatophytes. Natural Product Communications, 3(9): 1543–1546.
Iranshahy M. and Iranshahi M. 2011. Traditional uses, phytochemistry and pharmacology of asafoetida (Ferula assa-foetida oleo-gum-resin)- A review. Journal of Ethnopharmacology, 134: 1–10.
Irwandi J., Faridayanti S., Mohamed E., Hamzah M., Torla H. and Che Man Y. 2009. Extraction and characterization of gelatin from different marine fish species in Malaysia. International Food Research Journal, 16: 381–389.
Jamilah B. and Harvinder K. 2002. Properties of gelatins from skins of fish-black tilapia (Oreochromis mossambicus) and red tilapia (Oreochromis nilotica). Food Chemistry, 77: 81–84.
Jongjareonrak A., Benjakul S., Visessanguan W. and Tanaka M. 2006. Skin gelatin from bigeye snapper and brownstripe red snapper: Chemical compositions and effect of microbial transglutaminase on gel properties. Food Hydrocolloids, 20: 1216–1222.
Kajimoto T., Yahiro K. and Nohara T. 1989. Sesquiterpenoid and disulphide derivatives from Ferula assa-foetida. Phytochemistry, 28: 1761–1763.
Kamoun E.A., Kenawy E.R.S., Tamer T.M., El-Meligy M.A. and Eldin M.S.M. 2015. Poly (vinyl alcohol)-alginate physically crosslinked hydrogel membranes for wound dressing applications: Characterization and bio-evaluation. Arabian Journal of Chemistry, 8: 38–47.
Karim A. and Bhat R. 2009a. Extraction and characterization of gelatin from Atlantic salmon (Salmo salar) skin. Food Hydrocolloids, 23: 563–576.
Karim A. and Bhat R. 2009b. Fish gelatin: Properties, challenges, and prospects as an alternative to mammalian gelatins. Food Hydrocolloids, 23: 563–576.
Kavoosi G. and Rowshan V. 2013. Chemical composition, antioxidant and antimicrobial activities of essential oil obtained from Ferula assa-foetida oleo-gum-resin: Effect of collection time. Food Chemistry, 138: 2180–2187.
Lavorgna M., Piscitelli F., Mangiacapra P. and Buonocore G.G. 2010. Study of the combined effect of both clay and glycerol plasticizer on the properties of chitosan films. Carbohydrate Polymers, 82: 291–298.
Li Z., Lin S., An S., Liu L., Hu Y. and Wan L. 2019. Preparation, characterization and anti-aflatoxigenic activity of chitosan packaging films incorporated with turmeric essential oil. International Journal of Biological Macromolecules, 131: 420–434.
Limpisophon K., Tanaka M., Weng W., Abe S. and Osako K. 2009. Characterization of gelatin films prepared from under-utilized blue shark (Prionace glauca) skin. Food Hydrocolloids, 23: 1993–2000.
Lin L., Regenstein J.M., Lv S., Lu J. and Jiang S. 2017. An overview of gelatin derived from aquatic animals: Properties and modification. Trends in Food Science and Technology, 68: 102–112.
Mahendra P. and Bisht S. 2012. Ferula asafoetida: Traditional uses and pharmacological activity. Pharmacognosy Reviews, 6(12): 141–146.
Malafaya P.B., Silva G.A. and Reis R.L. 2007. Natural–origin polymers as carriers and scaffolds for biomolecules and cell delivery in tissue engineering applications. Advanced Drug Delivery Reviews, 59: 207–233.
Mathew S. and Abraham T.E. 2008. Characterisation of ferulic acid incorporated starch–chitosan blend films. Food Hydrocolloids, 22: 826–835.
Mirzapour Kouhdasht A., Moosavi Nasab M. and Aminlari M. 2018. Investigation of characteristics of gelatin produced from common carp (Cyprinus carpio) wastes by enzymatic hydrolysis. Journal of Nutrition Sciences and Food Technology, 13(1): 105–113.
Mu C., Li X., Zhao Y., Zhang H., Wang L. and Li D. 2013. Freezing/thawing effects on the exfoliation of montmorillonite in gelatin‐based bionanocomposite. Journal of Applied Polymer Science, 128: 3141–3148.
Muyonga J.H., Cole C.G.B. and Duodu K.G. 2004. Extraction and physico-chemical characterisation of Nile perch (Lates niloticus) skin and bone gelatin. Food Hydrocolloids, 18(4): 581–592.
Nuvoli L., Conte P., Fadda C., Ruiz J.A.R., Garcia J.M., Baldino S. and Mannu A. 2021. Structural, thermal, and mechanical properties of gelatin-based films integrated with tara gum. Polymer, 214: 123244.
Santoro M., Tatara A.M. and Mikos A.G. 2014. Gelatin carriers for drug and cell delivery in tissue engineering. Journal of Controlled Release, 190: 210–218.
Shaw G.S. 2016. Preparation and characterization of gelatin-tamarind gum/carboxymethyl tamarind gum based phase separated hydrogels and films for tissue engineering application. M.Sc. Thesis, National Institute of Technology Rourkela, India. 94P.
Soares R., Lima A., Oliveira R., Pires A. and Soldi V. 2005. Thermal degradation of biodegradable edible films based on xanthan and starches from different sources. Polymer Degradation and Stability, 90: 449–454.
Tinrat S. and Sila-Asna M. 2017. Optimization of gelatin extraction and physico-chemical properties of fish skin and bone gelatin: Its application to Panna cotta formulas. Current Research in Nutrition and Food Science Journal, 5: 263–273.
Torabi Ardekani N., Khorram M., Zomorodian K., Yazdanpanah S., Veisi H. and Veisi H. 2019. Evaluation of electrospun poly (vinyl alcohol)-based nanofiber mats incorporated with Zataria multiflora essential oil as              potential wound dressing. International Journal of Biological Macromolecules, 125: 743–750.
Wasswa J., Tang J. and Gu X. 2007. Utilization of fish processing by-products in the gelatin industry. Food Reviews International, 23: 159–174.
Xu S., Sun T., Xu Q., Duan C., Dai Y., Wang L. and Song Q. 2018. Preparation and antibiofilm properties of zinc oxide/porous anodic alumina composite films. Nanoscale Research Letters, 13: 1–12.
Xue F., Gu Y., Wang Y., Li C. and Adhikari B. 2019. Encapsulation of essential oil in emulsion based edible films prepared by soy protein isolate-gum acacia conjugates. Food Hydrocolloids, 96: 178–189.
Zomorodian K., Saharkhiz J., Pakshir K., Immeripour Z. and Sadatsharifi A. 2018. The composition, antibiofilm and antimicrobial activities of essential oil of Ferula assa-foetida oleo-gum-resin. Biocatalysis and Agricultural Biotechnology, 14: 300–304.