In vitro approach to determine oocyte maturation in beluga sturgeon (Huso huso)

Document Type : Research Paper

Authors

1 Professor in Fisheries Department, Faculty of Natural Resource, University of Guilan, Sowmeh Sara, Iran.

2 Professor in Department of Marine Sciences, The Caspian Sea Basin Research Center, University of Guilan, Rasht, Iran

3 M.Sc. in Fisheries, Shahid Dr. Beheshti Sturgeon Fishes Restoration and Genetic Conservation Complex, Sangar, Guilan, Iran

Abstract

Due to the high importance of beluga sturgeon breeders and to determine the appropriate time for hormonal induction, the present study was performed with the aim of selecting the breeders with high quality eggs for artificial reproduction. Two groups of eggs from 7 female beluga were incubated with 5 µg of progesterone per mL or hormone-free group in Ringer medium at 16°C for 18 hours. Pre- and post-exposure, the germinal vesicle migration (GVM) index was determined in 10 oocytes. The results showed that the GV position was 13.0±3.4 pre-treatment and changed after in vitro culture of oocytes in control and progesterone groups to 11.7±4.1 and 9.6±2.2, respectively, which showed a significant difference. During this period, the GVM exposed to progesterone was determined to be 31.2% closer to the animal pole. This study suggests that due to high economic value of beluga breeders, this method can be used to select suitable fish to induce artificial reproduction.

Keywords

References

طعنه ب.، ابطحی ب. و نظری ر.م. 1391. بررسی شاخص رسیدگی نهایی تخمک (GVBD) در تاس­ماهی ایرانی در شرایط In vitro به عنوان معیار انتخاب مولد. زیست‌شناسی جانوری تجربی، 1: 43-33.
فلاحتکار ب. 1395. دستور العمل مراکز تکثیر و پرورش ماهیان خاویاری (ترجمه). انتشارات آموزش تحقیقات کشاورزی. 328ص.
فلاحتکار ب. و عفت‌پناه ا. 1395. بیولوژی و فیزیولوژی ماهیان خاویاری دریای خزر. انتشارات دانشگاه گیلان. 247ص.
فلاحتکار ب.، خدابخشی ل. و فرامرزپور س. 1397. روند تغییرات استروئیدهای جنسی و شاخص‌های بیوشیمیایی خون استرلیاد پرورشی (Acipenser ruthenus) طی مرحله جذب تخمک. مجله پژوهش­های جانوری، 31:           105-93.
Chapman F.A. and Van Eenennaam J.P. 2007. Sturgeon aquaculture- specialized techniques determining the stage of sexual maturity in female sturgeon for artificial spawning: The egg polarization index or PI. IFAS Extension, University of Florida. P: 1–4 (FA153).
Conte F.S., Doroshov S.I., Lutes P.B. and Strange E.M. 1988. Hatchery Manual for the White Sturgeon (Acipenser transmontanus Richardson): With Application to Other North American Acipenseridae. UCANR Publications, USA. 104P.
Dettlaff T.A., Ginsburg A.S. and Schmalhausen O.I. 1993. Sturgeon Fishes: Developmental Biology and Aquaculture. Springer, Germany. 300P.
Doroshov S.I. and Lutes P.B. 1984. Preliminary data on the induction of ovulation in the white sturgeon (Acipenser transmontanus Richardson). Aquaculture, 38: 221–227.
Falahatkar B. and Poursaeid S. 2013. Stress responses of great sturgeon Huso huso subjected to husbandry stressors. Aquaculture International, 21: 947–959.
Falahatkar B., Akhavan S.R., Abbasalizadeh A. and Tolouei M.H. 2013. Sex identification and sexual maturity stages in farmed great sturgeon, Huso huso L. through biopsy. Iranian Journal of Veterinary Research, 14: 133–139.
Falahatkar B., Poursaeid S., Shakoorian M. and Barton B. 2009. Responses to handling and confinement stressors in juvenile great sturgeon Huso huso. Journal of Fish Biology, 75: 784–796.
Goncharov B.F. 2002. In vitro approach to studying the mechanisms of oocyte maturation in sturgeons: A review of fundamental and applied aspects. Journal of Applied Ichthyology, 18: 368–374.
IUCN. 2020. Red List of Threatened Species. Retrieved December 01, 2020, from www.iucnredlist.org.
Lutes P.B. 1985. Oocyte maturation in white sturgeon, Acipenser transmontanus: Some mechanisms and applications. P: 87–92. In: Binkowski F.P. and Doroshov S.I. (Eds.). North American Sturgeons: Biology and Aquaculture Potential. Springer, Netherlands.
Lutes P.B., Doroshov S.I., Chapman E., Harrah J., Fitzgerald R. and Fitzpatrick M. 1987. Morpho-physiological predictors of ovulatory success in white sturgeon, Acipenser transmontanus Richardson. Aquaculture, 66: 43–52.
Mojazi Amiri B., Maebayashi M., Omoto N., Adachi S. and Yamauchi K. 2001. In vitro oocyte maturation in a hybrid sturgeon, Bester: Changes in the germinal vesicle breakdown and 17,20β- dihydroxy-4-pregnen-3-one production. Journal of Agriculture Science and Technology, 3: 199–207.
Webb M.A.H., Van Eenennaam J.P. and Doroshov S.I. 2000. Effects of steroid hormones on in vitro oocyte maturation in white sturgeon (Acipenser transmontanus). Fish Physiology and Biochemistry, 23: 317–325.
Williot P. 1997. Effects of incubation media on maturation of isolated ovarian follicles of Siberian sturgeon (Acipenser baeri Brandt) induced by sturgeon gonadotropic preparation or 17a,20b-dihydroxyprogesterone. Comparative Biochemistry and Physiology (C), 118: 285–293.
Williot P. 2018. In vitro incubation of ovarian follicles of cultured Siberian sturgeon, Acipenser baerii Brandt: A short practical implementation and its fundamentals. P: 519–528. In: Williot P., Nonnotte G., Vizziano-Cantonnet D. and Chebanov M. (Eds.). The Siberian Sturgeon (Acipenser baerii, Brandt, 1869), Vol. 2: Farming. Springer, Switzerland.
Williot P., Brun R., Rouault T. and Rooryck O. 1991. Management of female spawners of Siberian sturgeon, Acipenser baeri Brandt: First results. P: 365–379. In: Williot P. (Ed.). Acipenser. Cemagref Publication, France.
Aquatic Physiology and Biotechnology
Volume 10, Issue 1
June 2022
Pages 21-35

Files

Share

How to cite

Statistics