Qualitative and quantitative analyze of PAHs (sixteen of important PAHs in EPA list) in tissues and meal of Clupeonella cultriventris caspia of Guilan Province Caspian waters (Bandar Anzali)



In present study common Klika from Guilan province coastal area (Bandar–e- Anzali) and fish meal produced from that, has been examined to investigate types and amount of Polycyclic aromatic hydrocarbons (PAHs). Bio-monitoring of pollutants is very important because they are carcinogenic and mutagen components, if they enter human food chain. Samples were collected from Anzali fishing port. 300Kg of fish sample sent to fish meal producing plant after fish containers were coded. 2 Kg of randomly selected sample was sent to laboratory for biometric analysis including fork length, weight and age determination by inner ear otolith method. For measuring PAHs, frozen samples were eventually taken to the laboratory and out of 16 PAH's compounds in EPA table for 3 year old fishes, 6 compounds were found namely: Naphthalen, Indeno (1,2,3…cd) pyrene, Pyrene, Fluorene, Anthracene and Dibanzo (ah) anthracene with quantity of 90.67, 19.75, 8.42, 5.93, 3.30 and 0.37 ppb respectively. In 5 years old fishes 74.00, 14.25 and 3.96 ppb Naphthalen, Indeno (1,2,3…cd) pyrene and Fluorene were determined respectively. While for 7 years old fishes no PHA has been found. For all age combinations 2 compounds i.e. Naphthalen and Indeno (1,2,3…cd) pyrene were present found with amount of 17.67 and 8.97 ppb respectively. In fish meal sample only Naphthalen and Indeno (1,2,3…cd) pyrene were recorded with amount of 11.73 and 5.98 ppb in order. The results also show that there is a significant difference between age increase and decline of Naphthalen, Pyrene, Indeno (1,2,3…cd) pyrene and Dibanzo (ah) anthracene. However there are no significances found between meal texture and produced meal in spite of decreasing trend. The results also showed that PAH amounts both in fresh Kilka and fish meal fortunately are below standard levels.